资料摘要
资料下载1动物实验 1.1跳台实验 1.1.1原理 反应箱底铺有通36v电的铜栅,动物受到电击,其正常反应是跳上箱内绝缘的平台以避免伤害性刺激。多数动物可能再次或多次跳至铜栅上,受到电击又迅速跳回平台,如此训练5min,并记录每鼠受到电击的次数或叫错误次数,以此作为学习成绩。24h或48h重作测验,此即记忆保持测验。记录受电击的动物数、第一次跳下平台的潜伏期和3min内的错误总数。停止训练5天后(也可以在训练后的一周、两周或其它时间点)进行记忆消退实验。 1.1.2仪器与试剂 跳台仪:该装置为10×10×60cm3的被动回避条件反射箱,用黑色塑料板分隔成5间。底面铺以铜栅,间距为0.5cm,可以通电,电压强度由一变压器控制。每间左后角置一高和直径均为4.5cm的绝缘平台。 试剂:樟柳碱、环已酰亚胺、乙醇。 1.1.3实验方法 1.1.3.1实验动物 断乳鼠,体重10g左右;或成年鼠,体重18-22g用于改善老年人记忆的产品必须采用成年鼠。雌雄均可,单一性别不少于10只(最后有效数据)。推荐使用近交系小鼠。 1.1.3.2剂量设计和分组 共设高、中、低、对照四个组,根据推荐的人体每日每公斤体重摄入量,扩大10倍作为其中一个剂量组,根据受试样品的具体情况另设两个剂量组。经口给样。原则上连续给样30天,也可根据实验需要自行设定给样期限。
斑马鱼胚胎显微注射实验操作方法
简介:斑马鱼显微注射是将实验材料直接注射到1-4细胞期的斑马鱼胚胎中,由于在这个胚胎发育早期没有膜分隔细胞和卵黄,注入1个细胞或卵黄的溶液将扩散到整个胚胎中。通过注射不同类型的实验样品,实现基因的瞬时过表达(over-expression)、表达敲减(knock-down)、以及制备转基因或突变斑马鱼品系等研究。
桌式麻醉机使用说明
简介:吸入式气体麻醉,是在供给动物的气流中添加入麻醉气体(浓度受麻醉挥发罐的精确控制,且可随动物生理状况实时调节),以此对动物进行麻醉。 在维持麻醉过程前,需要以高麻醉浓度、大流量的气流对动物进行诱导麻醉,方便对动物进行固定处理等术前准备。 麻醉尾气需通入活性炭盒(F/AIR)进行吸收处理,以消除其对实验人员的影响。
脑立体定位仪的使用方法
简介:脑立体定位仪是利用颅骨外面的标志或其他参考点所规定的三维坐标系统来确定皮层下某些神经结构的位置,以便在非直视暴露下对其进行定向的刺激、破坏、注射药物或引导电位等操作。 它是神经解剖、神经生理、神经药理和神经外科等领域内重要的研究设备,可用于帕金森氏病动物模型的建立、电线动物模型的建立、脑内肿瘤动物模型的建立、学习记忆、神经干细胞移植、脑缺血等研究。
Mixture/Dose Delivery System
简介:The PHD ULTRA? Gradient Systems allows you to quickly and easily run binary or ternary mixtures automatically without a PC. The Method is totally programmed from a master controller pump, for easy set-up. The intuitive program interface makes simple to complex mixing easy. The Gradient Profile is used to combine multiple flow streams from different pumps into a common output stream. The flow can be smooth and continuous or stepped. No stopping the experiment, mixing different % volume mixtures and placing new syringes into the pumps. Push one button and it all happens automatically. Binary and ternary gradient systems are available with or without a stand. Individual Satellite units are also available for purchase separately. For more information on the PHD ULTRA? Syringe Pumps see page 18-22. For more information on the PHD ULTRA? Satellite units see page 21.
harvard pump 11 pico plus使用手...
简介:General Information Serial Number All inquires concerning our product should refer to the serial number of the unit. Serial numbers are located on the rear of the chassis. Calibration All syringe pumps are designed and manufactured to meet their performance specifications at all rated voltages and frequencies. A calibration certificate is available upon request. Contact customer service for details and pricing. Warranty Harvard Apparatus warranties this instrument for a period of two year from date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material. This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to the original customer purchaser. IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied warranty, so the above limitation may not apply to you.
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