APP antibody

APP antibody
Cat.#: 1565-1
Rabbit Monoclonal Antibody
Clone ID: Y188
Swiss Prot: P05067
Mol Weight: 95kDa
Size: 100ul

Description
Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (Aβ4) protein has been found to be the principal constituent of senile plaques of ADs patients (1-2). APP is proteolytically cleaved to generate the 4 kDa Aβ4 protein. Two variants of Aβ4 can be detected; Aβ1-40 short-tailed and Aβ1-42 long-tailed (3-4).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:250-500
ICC: 1:250-500
IP: 1:50
FC: 1:20

Species Reactivity
Human, Mouse, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data  

 

A. Western blot analysis on Hela cell lysate using anti-APP RabMAb (cat. # 1565-1), dilution 1:20,000
Staining of various paraffin-embedded human tissue samples using anti-APP RabMAb (cat#1565-1). Liver showed negative staining. Tissues were stained in parallel on the same Normal Tissue Array.
Staining of various paraffin-embedded human tissue samples using anti-APP RabMAb (cat#1565-1). Skeletal muscle showed negative staining. Tissues were stained in parallel on the same Normal Tissue Array.
 

Staining of various paraffin-embedded human tissue samples using anti-APP RabMAb (cat#1565-1). Human brain tissue showed positive immunohistochemical staining for Amyloid Beta A4 precursor. Tissues were stained in parallel on the same Normal Tissue Array.
Staining of various paraffin-embedded human tissue samples using anti-APP RabMAb (cat#1565-1). Human breast showed negative staining. Tissues were stained in parallel on the same Normal Tissue Array.

Specificity
A synthetic peptide corresponding to residues in the NPXY motif of human APP was used as immunogen. This antibody specifically recognizes APP and its isoforms. It will not recognize either of the two Amyloid Beta Protein ( Aβ4 ) variants, Aβ1 -40 or Aβ1 -42.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APP antibody, AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN2 antibody, C31 antibody, Gamma-CTF(50) antibody

Description References
1. Glenner, G. G., Wong, C. W. (1984) Biochem. Biophys. Res. Commun. 120, 885-890
2. Masters, C. L., Simms, G., Weinman, N. A., Multhaup, G., McDonald, B., Beyreuther, K. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 4245-4249
3. Dovey, H. F., Suomesaari-Chrysler, S., Lieberburg, I., Sinha, S., and Kiem, P. S. (1993) NeuroReport 4, 1039-1042
4. Vigo-Pelfrey, C., Lee, D., Keim, P., Lieberburg, I., Schenk, D. B. (1993) J. Neurochem. 61, 1965-1968

Product References
1. Barbagallo AP, Wang Z, Zheng H, et al, A Single Tyrosine Residue in the Amyloid Precursor Protein Intracellular Domain Is Essential for Developmental Function ,J Biol Chem 286 (2011) 8717-8721
2. Wang ZL, Wang BP, Yang L, et al, Presynaptic and Postsynaptic Interaction of the Amyloid Precursor Protein Promotes Peripheral and Central Synaptogenesis ,J. Neurosci. 29 (2009) 10788 - 10801
3. Li H, Wang B, Wang Z, et al, Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP ,Proc Natl Acad Sci U S A 107 (2010) 17362-17367