Alpha Actinin antibody

Alpha Actinin antibody

参考价:¥2890
供货周期: 一周
品牌:
规格: 100ul
货号: S1429
CAS号:
上海希美化学有限公司
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Alpha Actinin antibody
Cat.#: S1429
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P12814
Mol Weight: 103.08kDa
Size: 100ul

Description
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:500-1:3000

Species Reactivity
Human

Other species have not been tested

Products Data 

 


Sample(30ug whole cell lysate)
A: 293T  B: A431   C: H1299  D: HeLa S3   E: Hep G2   F: MOLT4   G: Raji     7.5% SDS PAGE
diluted at 1:1000 Sample (30ug of whole cell lysate) A:NIH-3T3  7.5% SDS PAGE diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1 antibody at 1:100 dilution.

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 220 and 350 of Human ACTN1

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
ACTN1 antibody, FLJ40884 antibody, FLJ54432 antibody, Alpha-actinin-1 antibody, Alpha-actinin cytoskeletal isoform antibody, F-actin cross-linking protein antibody, Non-muscle alpha-actinin-1 antibody

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