Mouse Anti Glutathione-S-transferase (GST)

Mouse Anti Glutathione-S-transferase (GST)

参考价：¥2170
供货周期： 一周
品牌：
规格： 100µg
货号： ANT-219
CAS号：
上海希美化学有限公司
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Catalogue Number ANT-219
Type Mouse Antibody Monoclonal.
Introduction GST family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST tag has the size of 220 amino acids, which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminus of a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins.
Clone PGSTSHG.
Description Monoclonal antibodies are produced by immunizing mouse with GST protein.
Ig Subclass Mouse IgG2b.
Formulation 1×PBS & 50% glycerol.
Stability / Shelf Life 2 years at -20°C. 1 month at 4°C.
Dilution Western Blotting: 1μg/ml.
Applications Western Blot, Immunoprecipitation.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Mouse Anti Glutathione-S-transferase (GST)由上海希美化学有限公司为您提供，货号ANT-219，规格：100µg，CAS号：，如您想了解更多关于Mouse Anti Glutathione-S-transferase (GST)价格、Mouse Anti Glutathione-S-transferase (GST)结构式、批发、用途等信息，欢迎咨询。除供应Mouse Anti Glutathione-S-transferase (GST)外，还可为您提供重组谷胱甘肽-S-转移酶(GST)、小鼠α谷胱甘肽S转移酶(α-GST)ELISA Kit等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。
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