Mouse Anti Glutathione-S-transferase (GST)|500µg|ANT-164-上海希美化学有限公司

产品详情

Catalogue Number

ANT-164

Type

Mouse Antibody Monoclonal.

Introduction

GST family of enzymescomprises a long list of cytosolic, mitochondrial, and microsomalproteinsthat are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenousand xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST tag has the size of 220 amino acids, which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminusof a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombindomain for cleavage of the GST tag during protein purification. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins.

Clone

6C7.

Immunogen

glutathione-S-transferase (GST) of Schistosoma japonicum.

Ig Subclass

Mouse IgG1.

Purification Method

Protein A column.

Protein concentration

Lyophilized with no salts.

Stability / Shelf Life

Ten years lyophilized. One month in solution stored at 4°C.

Reconstitution

Reconstitute with 1.0 ml of PBS. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Protein after reconstitution is 2 mgs/ml.

Specificity

glutathione-S-transferase (GST) of Schistosoma japonicum.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Mouse Anti Glutathione-S-transferase (GST)由上海希美化学有限公司为您提供，货号ANT-164，规格：500µg，CAS号：，如您想了解更多关于Mouse Anti Glutathione-S-transferase (GST)价格、Mouse Anti Glutathione-S-transferase (GST)结构式、批发、用途等信息，欢迎咨询。除供应Mouse Anti Glutathione-S-transferase (GST)外，还可为您提供重组谷胱甘肽-S-转移酶(GST)、小鼠α谷胱甘肽S转移酶(α-GST)ELISA Kit等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

Business information

工商信息

信息已认证

企业名称: 上海希美化学有限公司
企业类型:
信用代码: 310228001203103
成立日期: 2009年05月31日
注册资本: 10
经营范围: 化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】

供货周期：	一周
品牌：
规格：	500µg
货号：	ANT-164
CAS号：