Polyclonal Rabbit Anti Glutathione-S-transferase (GST) Affinity Purified|20µg|ANT-190-上海希美化学有限公司

产品详情

Catalogue Number

ANT-190

Type

Polyclonal Rabbit Antibody.

Introduction

GST family of enzymescomprises a long list of cytosolic, mitochondrial, and microsomalproteinsthat are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenousand xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST tag has the size of 220 amino acids, which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminusof a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombindomain for cleavage of the GST tag during protein purification. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins.

Description

Polyclonal antibody to recombinant glutathione-S-transferase (GST) was raised by immunizing rabbits with affinity purified GST. The protein was expressed in bacteria and purified by glutathione-affinity chromatography. From the crude serum, antigen-specific antibodies were purified by antigen-affinity chromatography. The product is provided as antigen-affinity purified IgG, with 0.05 % sodium azide as a preservative.

Specificity

The monospecific polyclonal IgG antibody against GST recognizes recombinant GST. It reacts specifically to a variety of GST fusion proteins used as targets in immunoblotting experiments. It also immunoprecipitates the target proteins efficiently. This antibody is reactive with immobilized native antigens on ELISA plates as well.

Uses

Polyclonal antibody against GST may be used for the capturing of GST fusion proteins using various immunochemical assays including immunobotting, dot blotting, immunoprecipitation, ELISA and immunohistochemistry (immunofluorescence or immuno-enzymatic staining).

Storage

For continuous use, store at 2-8°C. For extended storage freeze, in desirable aliuots. Repeated freezing and thawing is not recommended.

Concentration

1mg/1ml .

Recommended Dilution

Western Blotting: 1/10,000
ELISA: 1/200,000.

Note

The above dilution may vary with different GST fusion proteins. Therefore, the optimal working dilution may have to be determined for best results.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Polyclonal Rabbit Anti Glutathione-S-transferase (GST) Affinity Purified由上海希美化学有限公司为您提供，货号ANT-190，规格：20µg，CAS号：，如您想了解更多关于Polyclonal Rabbit Anti Glutathione-S-transferase (GST) Affinity Purified价格、Polyclonal Rabbit Anti Glutathione-S-transferase (GST) Affinity Purified结构式、批发、用途等信息，欢迎咨询。除供应Polyclonal Rabbit Anti Glutathione-S-transferase (GST) Affinity Purified外，还可为您提供重组谷胱甘肽-S-转移酶(GST)、小鼠α谷胱甘肽S转移酶(α-GST)ELISA Kit、Polyclonal Rabbit Anti Human Heparanase-1 （HPA-1）等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

供货周期：	一周
品牌：
规格：	20µg
货号：	ANT-190
CAS号：