Mouse Anti Maltose-Binding protein （MBP）

Mouse Anti Maltose-Binding protein （MBP）

参考价：¥3500
供货周期： 一周
品牌：
规格： 20µg
货号： ANT-177
CAS号：
上海希美化学有限公司
银牌会员 丨 13年 丨 生产商
关注展位 全部试剂
产品详情

Catalogue Number ANT-177
Type Mouse Antibody Monoclonal.
Introduction Maltose Binding Protein (MBP) is a part of the maltose/maltodextrin system of Eschericia coli bacteria, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes.
Maltose binding protein MalE,MBP, is part of the maltodextrin transport system in E. coli. The maltodextrins diffuse through the LamB pore located in the outer membrane into the periplasm. The sugar is bound to MalE which prevents it to diffuse back outside. MalE passes the sugar to a complex of MalF and MalG in the cytoplasmic membrane where active tranport is mediated by the ATPase MalK. MBP molecular weight is 40,622 dalton. There are two domains in the protein with similar secondary structure (central pleated sheets are surrounded by helices) . The domains are connected by three bridges, between the domains is a deep groove in which the sugar is bound. All hydroxyl groups of the sugar are fixed by sidechains of the protein by hydrogen bonds. Some water molecules are part of the hydrogen bridge network.
Specificity Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).
Antigen used for immunization The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109.
The purified recombinant MBP protein was then used for mouse immunization.
Storage Buffer Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.
Recommendations on use Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25°C.
Westren Blotting Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25°C.
Storage and Stability Store unopened antibody at 4°C or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4°C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20°C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» MBP Antibody
» MBP E.Coli

相关试剂 更多

相关产品

同类推荐 更多
Mouse Anti Maltose-Binding protein （MBP）由上海希美化学有限公司为您提供，货号ANT-177，规格：20µg，CAS号：，如您想了解更多关于Mouse Anti Maltose-Binding protein （MBP）价格、Mouse Anti Maltose-Binding protein （MBP）结构式、批发、用途等信息，欢迎咨询。除供应Mouse Anti Maltose-Binding protein （MBP）外，还可为您提供Mouse Anti Human C-Reactive Protein （CRP）、Mouse Anti Human Cellular Retinoic Acid binding Protein 2 （CRABP2）等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。
Business information
工商信息 信息已认证
contact us
联系方式

详细地址

上海市松江区陆家圈路168弄113号
当前位置： 上海希美化学 试剂 Mouse Anti Maltose-Binding protein （MBP）

关注

拨打电话

留言咨询