供货周期: | 一周 |
品牌: | |
规格: | 10ug |
货号: | cyt-041 |
CAS号: |
人双调蛋白
Recombinant Human Amphiregulin(AREG)
Catalogue Number |
CYT-041 |
Synonyms |
|
Introduction |
Amphiregulin (AREG) belongs to the EGF family of cytokines that contain 10 proteins such as EGF, TGFb, HBEGF, and the various heregulins. These cytokines are synthesized as transmembrane precursors and are categorized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Initially, Amphiregulin (AREG) was isolated from the conditioned media of a PMA treated MCF 7 human breast carcinoma cell line. Multiple forms of native AR containing either 78 or 84 amino acid residues and both N and O-linked oligosaccharides have been found. Amphiregulin (AREG) mRNA expression can be identified in several carcinoma cell lines and the epithelial cells of numerous human tissues such as colon, stomach, breast, ovary, kidney, etc. |
Description |
Amphiregulin (AREG) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and having a molecular mass of 11.3 KDa. The AREG is purified by proprietary chromatographic techniques. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4. |
Solubility |
|
Stability |
|
Purity |
Greater than 95.0% as determined by (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. |
Amino acid sequence |
SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK. |
Biological Activity |
Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml. |
Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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