供货周期: | 一周 |
品牌: | |
规格: | 96T |
货号: | CSB-E13927h |
CAS号: | CSB-E13927h |
产品名称: 人甲状腺激素受体β(THRb)ELISA Kit
英文名称: Human Thyroid Hormone Receptor β (THRb) ELISA Kit
货号: CSB-E13927h
规格: 96T
种属: Human
待测物名称: thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
缩写: THRB
蛋白功能1: Transcription/Transcription regulation
蛋白功能3: Transcription
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for THRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any THRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for THRB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of THRB bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human THRB. No significant cross-reactivity or interference between Human THRB and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the THRB concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
相关产品:
产品名称 英文名称 货号 规格
Sheep Thyroid hormone receptor beta(THRB) ELISA kit Sheep Thyroid hormone receptor beta(THRB) ELISA kit CSB-EL023514SH 96T
Rat Thyroid hormone receptor beta(THRB) ELISA kit Rat Thyroid hormone receptor beta(THRB) ELISA kit CSB-EL023514RA 96T
Mouse Thyroid hormone receptor beta(THRB) ELISA kit Mouse Thyroid hormone receptor beta(THRB) ELISA kit CSB-EL023514MO 96T
Duck Thyroid hormone receptor beta(THRB) ELISA kit Duck Thyroid hormone receptor beta(THRB) ELISA kit CSB-EL023514MU 96T
Chicken Thyroid hormone receptor beta(THRB) ELISA kit Chicken Thyroid hormone receptor beta(THRB) ELISA kit CSB-EL023514CH 96T
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Leuconostoc citreum Homoserine kinase(thrB) CSB-EP023514LPD 1mg E.coli
Recombinant Leuconostoc citreum Homoserine kinase(thrB) CSB-YP023514LPD 1mg Yeast
Recombinant Yersinia pseudotuberculosis serotype O:3 Homoserine kinase(thrB) CSB-EP023514YAX 1mg E.coli
Recombinant Yersinia pseudotuberculosis serotype O:3 Homoserine kinase(thrB) CSB-YP023514YAX 1mg Yeast
Recombinant Cyanothece sp. Homoserine kinase(thrB) CSB-EP023514DZY 1mg E.coli
Recombinant Cyanothece sp. Homoserine kinase(thrB) CSB-YP023514DZY 1mg Yeast
Recombinant Escherichia coli Homoserine kinase(thrB) CSB-EP023514ENX 1mg E.coli
Recombinant Escherichia coli Homoserine kinase(thrB) CSB-YP023514ENX 1mg Yeast
Recombinant Corynebacterium urealyticum Homoserine kinase(thrB) CSB-EP023514DXD 1mg E.coli
Recombinant Corynebacterium urealyticum Homoserine kinase(thrB) CSB-YP023514DXD 1mg Yeast
Recombinant Methylobacterium radiotolerans Homoserine kinase(thrB) CSB-EP023514MTD 1mg E.coli
Recombinant Methylobacterium radiotolerans Homoserine kinase(thrB) CSB-YP023514MTD 1mg Yeast
Recombinant Burkholderia cenocepacia Homoserine kinase(thrB) CSB-EP023514BXQ 1mg E.coli
Recombinant Burkholderia cenocepacia Homoserine kinase(thrB) CSB-YP023514BXQ 1mg Yeast
Recombinant Clostridium botulinum Homoserine kinase(thrB) CSB-EP023514DUI 1mg E.coli
Recombinant Clostridium botulinum Homoserine kinase(thrB) CSB-YP023514DUI 1mg Yeast
Recombinant Escherichia coli Homoserine kinase(thrB) CSB-EP023514ENT 1mg E.coli
Recombinant Escherichia coli Homoserine kinase(thrB) CSB-YP023514ENT 1mg Yeast
Recombinant Mycobacterium abscessus Homoserine kinase(thrB) CSB-EP023514MVF 1mg E.coli
Recombinant Mycobacterium abscessus Homoserine kinase(thrB) CSB-YP023514MVF 1mg Yeast
Recombinant Clostridium botulinum Homoserine kinase(thrB) CSB-EP023514DUJ 1mg E.coli
Recombinant Clostridium botulinum Homoserine kinase(thrB) CSB-YP023514DUJ 1mg Yeast
Recombinant Streptococcus pneumoniae Homoserine kinase(thrB) CSB-EP023514FNA 1mg E.coli
Recombinant Streptococcus pneumoniae Homoserine kinase(thrB) CSB-YP023514FNA 1mg Yeast
Recombinant Lysinibacillus sphaericus Homoserine kinase(thrB) CSB-EP023514LRV 1mg E.coli
Recombinant Lysinibacillus sphaericus Homoserine kinase(thrB) CSB-YP023514LRV 1mg Yeast
Recombinant Xylella fastidiosa Homoserine kinase(thrB) CSB-EP023514XBM 1mg E.coli
Recombinant Xylella fastidiosa Homoserine kinase(thrB) CSB-YP023514XBM 1mg Yeast
Recombinant Caulobacter sp. Homoserine kinase(thrB) CSB-EP023514DQR 1mg E.coli
Recombinant Caulobacter sp. Homoserine kinase(thrB) CSB-YP023514DQR 1mg Yeast
Recombinant Xanthomonas campestris pv. campestris Homoserine kinase(thrB) CSB-EP023514XAZ 1mg E.coli
Recombinant Xanthomonas campestris pv. campestris Homoserine kinase(thrB) CSB-YP023514XAZ 1mg Yeast
Recombinant Halobacterium salinarum Homoserine kinase(thrB) CSB-EP023514HTL 1mg E.coli
Recombinant Halobacterium salinarum Homoserine kinase(thrB) CSB-YP023514HTL 1mg Yeast
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询