供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-E17087h |
CAS号: | CSB-E17087h |
产品名称: 人极低密度脂蛋白(VLDL)ELISA Kit
英文名称: Human very low density lipoprotein(VLDL)ELISA Kit
货号: CSB-E17087h
规格: 96T
种属: Human
待测物名称: very low density lipoprotein(VLDL)
缩写: VLDL
样本类型: serum, plasma, tissue homogenates
检测范围: 0.78 μg/ml-50 μg/ml
灵敏度: 0.39 μg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the VLDL concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
相关产品:
产品名称 英文名称 货号 规格
小鼠极低密度脂蛋白(VLDL)ELISA Kit Mouse very low density lipoprotein(VLDL)ELISA Kit CSB-E17089m 96T
兔子Ⅰ型前胶原N端前肽(PⅠNP)ELISA Kit Rabbit procollagen Ⅰ N-terminal peptide(PⅠNP)ELISA Kit CSB-E17085Rb 96T
鸭极低密度脂蛋白(VLDL)ELISA Kit Duck very low density lipoprotein(VLDL)ELISA Kit CSB-EQ027316DU 96T
仓鼠极低密度脂蛋白(VLDL)ELISA Kit Hamster very low density lipoprotein(VLDL)ELISA Kit CSB-EQ027316HA 96T
猪白介素13(IL-13)ELISA Kit Pig Interleukin 13(IL-13) ELISA Kit CSB-E17086p 96T
小鼠垂草扁桃酸(VMA) ELISA Kit Mouse vanillylmandelic acid(VMA) ELISA Kit CSB-E17083m 96T
大鼠极低密度脂蛋白(VLDL)ELISA Kit Rat very low density lipoprotein(VLDL)ELISA Kit CSB-E17088r 96T
猪极低密度脂蛋白(VLDL)ELISA Kit Pig very low density lipoprotein(VLDL)ELISA Kit CSB-EQ027316PI 96T
鸡极低密度脂蛋白(VLDL)ELISA Kit Chicken very low density lipoprotein(VLDL)ELISA Kit CSB-E15984C 96T
兔子总碱性磷酸酶(TALP)ELISA Kit Rabbit total alkaline phosphatase(TALP)ELISA Kit CSB-E17084Rb 96T
马结合珠蛋白/触珠蛋白(Hpt/HP)ELISA Kit Horse Haptoglobin(Hpt/HP)ELISA Kit CSB-E17080Hs 96T
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询