供货周期: | 一周 |
品牌: | |
规格: | 96T |
货号: | CSB-E13659h |
CAS号: | CSB-E13659h |
产品名称: 人犬尿氨酸(KYN)ELISA Kit
英文名称: Human Kynurenine,KYN ELISA Kit
货号: CSB-E13659h
规格: 96T
种属: Human
待测物名称: kynureninase (L-kynurenine hydrolase)
缩写: KYNU
蛋白功能1: Biosynthesis/Metabolism
蛋白功能2: Alkaloid biosynthesis and metabolism
蛋白功能3: Pyridine nucleotide biosynthesis
样本类型: serum, urine and tissue homogenates
检测范围: 78 pmol/ml -5000 pmol/ml
灵敏度: 19.5 pmol/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KYNU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KYNU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KYNU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KYNU bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human KYNU. No significant cross-reactivity or interference between Human KYNU and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the KYNU concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
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产品名称 英文名称 货号 规格
Rat Kynureninase(KYNU) ELISA kit Rat Kynureninase(KYNU) ELISA kit CSB-EL012703RA 96T
Mouse Kynureninase(KYNU) ELISA kit Mouse Kynureninase(KYNU) ELISA kit CSB-EL012703MO 96T
抗体
产品名称 货号 规格
Rabbit anti-human kynureninase (L-kynurenine hydrolase) polyclonal Antibody CSB-PA012703GA01HU 150ul
蛋白
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Recombinant Stenotrophomonas maltophilia Kynureninase(kynU) CSB-YP012703FLX 1mg Yeast
Recombinant Bacillus weihenstephanensis Kynureninase(kynU) CSB-EP012703BON 1mg E.coli
Recombinant Bacillus weihenstephanensis Kynureninase(kynU) CSB-YP012703BON 1mg Yeast
Recombinant Monosiga brevicollis Kynureninase(kynu) CSB-EP012703MOD 1mg E.coli
Recombinant Monosiga brevicollis Kynureninase(kynu) CSB-YP012703MOD 1mg Yeast
Recombinant Kynureninase CSB-EP012703CXX 1mg E.coli
Recombinant Kynureninase CSB-YP012703CXX 1mg Yeast
Recombinant Nematostella vectensis Kynureninase(kynu) CSB-EP012703NGO 1mg E.coli
Recombinant Nematostella vectensis Kynureninase(kynu) CSB-YP012703NGO 1mg Yeast
Recombinant Bacillus cereus subsp. cytotoxis Kynureninase(kynU) CSB-EP012703BOK 1mg E.coli
Recombinant Bacillus cereus subsp. cytotoxis Kynureninase(kynU) CSB-YP012703BOK 1mg Yeast
Recombinant Vanderwaltozyma polyspora Kynureninase(BNA5) CSB-EP012703VDW 1mg E.coli
Recombinant Vanderwaltozyma polyspora Kynureninase(BNA5) CSB-YP012703VDW 1mg Yeast
Recombinant Sclerotinia sclerotiorum Kynureninase(bna5) CSB-EP012703STG 1mg E.coli
Recombinant Sclerotinia sclerotiorum Kynureninase(bna5) CSB-YP012703STG 1mg Yeast
Recombinant Botryotinia fuckeliana Kynureninase(bna5) CSB-EP012703BVD 1mg E.coli
Recombinant Botryotinia fuckeliana Kynureninase(bna5) CSB-YP012703BVD 1mg Yeast
Recombinant Flavobacterium psychrophilum Kynureninase(kynU) CSB-EP012703FDU 1mg E.coli
Recombinant Flavobacterium psychrophilum Kynureninase(kynU) CSB-YP012703FDU 1mg Yeast
Recombinant Flavobacterium johnsoniae Kynureninase(kynU) CSB-EP012703FDT 1mg E.coli
Recombinant Flavobacterium johnsoniae Kynureninase(kynU) CSB-YP012703FDT 1mg Yeast
Recombinant Lodderomyces elongisporus Kynureninase(BNA5) CSB-EP012703LLV 1mg E.coli
Recombinant Lodderomyces elongisporus Kynureninase(BNA5) CSB-YP012703LLV 1mg Yeast
Recombinant Meyerozyma guilliermondii Kynureninase(BNA5) CSB-EP012703MTM 1mg E.coli
Recombinant Meyerozyma guilliermondii Kynureninase(BNA5) CSB-YP012703MTM 1mg Yeast
Recombinant Salinispora tropica Kynureninase(kynU) CSB-EP012703STD 1mg E.coli
Recombinant Salinispora tropica Kynureninase(kynU) CSB-YP012703STD 1mg Yeast
Recombinant Magnaporthe oryzae Kynureninase(BNA5) CSB-EP012703MPM 1mg E.coli
Recombinant Magnaporthe oryzae Kynureninase(BNA5) CSB-YP012703MPM 1mg Yeast
Recombinant Geobacillus thermodenitrificans Kynureninase(kynU) CSB-EP012703GEH 1mg E.coli
Recombinant Geobacillus thermodenitrificans Kynureninase(kynU) CSB-YP012703GEH 1mg Yeast
Recombinant Burkholderia pseudomallei Kynureninase(kynU) CSB-EP012703BPT 1mg E.coli
Recombinant Burkholderia pseudomallei Kynureninase(kynU) CSB-YP012703BPT 1mg Yeast
Recombinant Burkholderia pseudomallei Kynureninase(kynU) CSB-EP012703BPU 1mg E.coli
Recombinant Burkholderia pseudomallei Kynureninase(kynU) CSB-YP012703BPU 1mg Yeast
Recombinant Burkholderia mallei Kynureninase(kynU) CSB-EP012703BPQ 1mg E.coli
Recombinant Burkholderia mallei Kynureninase(kynU) CSB-YP012703BPQ 1mg Yeast
Recombinant Scheffersomyces stipitis Kynureninase(BNA5) CSB-EP012703SXM 1mg E.coli
Recombinant Scheffersomyces stipitis Kynureninase(BNA5) CSB-YP012703SXM 1mg Yeast
Recombinant Burkholderia mallei Kynureninase(kynU) CSB-EP012703BPP 1mg E.coli
Recombinant Burkholderia mallei Kynureninase(kynU) CSB-YP012703BPP 1mg Yeast
Recombinant Burkholderia mallei Kynureninase(kynU) CSB-EP012703BPR 1mg E.coli
Recombinant Burkholderia mallei Kynureninase(kynU) CSB-YP012703BPR 1mg Yeast
Recombinant Bacillus thuringiensis Kynureninase(kynU) CSB-EP012703BOM 1mg E.coli
Recombinant Bacillus thuringiensis Kynureninase(kynU) CSB-YP012703BOM 1mg Yeast
Recombinant Gramella forsetii Kynureninase(kynU) CSB-EP012703GEQ 1mg E.coli
Recombinant Gramella forsetii Kynureninase(kynU) CSB-YP012703GEQ 1mg Yeast
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase(BNA5) CSB-EP012703CTL 1mg E.coli
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase(BNA5) CSB-YP012703CTL 1mg Yeast
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase(BNA5) CSB-EP012703CTK 1mg E.coli
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase(BNA5) CSB-YP012703CTK 1mg Yeast
Recombinant Kynureninase(kynU) CSB-EP012703FFT 1mg E.coli
Recombinant Kynureninase(kynU) CSB-YP012703FFT 1mg Yeast
Recombinant Rat Kynureninase(Kynu) CSB-EP012703RA 1mg E.coli
Recombinant Rat Kynureninase(Kynu) CSB-YP012703RA 1mg Yeast
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