供货周期: | 一周 |
品牌: | |
规格: | 96T |
货号: | CSB-E13844r |
CAS号: | CSB-E13844r |
产品名称: 大鼠细胞色素P450 1A1(CYP1A1)ELISA Kit
英文名称: Rat Cytochrome P450 1A1,CYP1A1 ELISA Kit
别名: AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX
货号: CSB-E13844r
规格: 96T
种属: Rat
待测物名称: cytochrome P450, family 1, subfamily A, polypeptide 1
缩写: CYP1A1
检测范围: 0.32 ng/ml-20 ng/ml
灵敏度: 0.08ng/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP1A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Rat CYP1A1. No significant cross-reactivity or interference between Rat CYP1A1 and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CYP1A1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
相关产品:
产品名称 英文名称 货号 规格
Fish Cytochrome P450 1A1(CYP1A1) ELISA kit Fish Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395FI 96T
Mouse Cytochrome P450 1A1(CYP1A1) ELISA kit Mouse Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395MO 96T
Sheep Cytochrome P450 1A1(CYP1A1) ELISA kit Sheep Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395SH 96T
Rabbit Cytochrome P450 1A1(CYP1A1) ELISA kit Rabbit Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395RB 96T
Monkey Cytochrome P450 1A1(CYP1A1) ELISA kit Monkey Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395RH 96T
Human Cytochrome P450 1A1(CYP1A1) ELISA kit Human Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395HU 96T
Guinea pig Cytochrome P450 1A1(CYP1A1) ELISA kit Guinea pig Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395GU 96T
Dog Cytochrome P450 1A1(CYP1A1) ELISA kit Dog Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395DO 96T
Cat Cytochrome P450 1A1(CYP1A1) ELISA kit Cat Cytochrome P450 1A1(CYP1A1) ELISA kit CSB-EL006395CA 96T
抗体
产品名称 货号 规格
Rabbit anti-human cytochrome P450, family 1, subfamily A, polypeptide 1 polyclonal Antibody CSB-PA006395GA01HU 150ul
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Liza aurata Cytochrome P450 1A1(cyp1a1) CSB-EP006395LQE 1mg E.coli
Recombinant Liza aurata Cytochrome P450 1A1(cyp1a1) CSB-YP006395LQE 1mg Yeast
Recombinant Sparus aurata Cytochrome P450 1A1(cyp1a1) CSB-EP006395FJX 1mg E.coli
Recombinant Sparus aurata Cytochrome P450 1A1(cyp1a1) CSB-YP006395FJX 1mg Yeast
Recombinant Limanda limanda Cytochrome P450 1A1(cyp1a1) CSB-EP006395LPP 1mg E.coli
Recombinant Limanda limanda Cytochrome P450 1A1(cyp1a1) CSB-YP006395LPP 1mg Yeast
Recombinant Rat Cytochrome P450 1A1(Cyp1a1) CSB-EP006395RA 1mg E.coli
Recombinant Rat Cytochrome P450 1A1(Cyp1a1) CSB-YP006395RA 1mg Yeast
Recombinant Macaca fascicularis Cytochrome P450 1A1(CYP1A1) CSB-EP006395MOV 1mg E.coli
Recombinant Macaca fascicularis Cytochrome P450 1A1(CYP1A1) CSB-YP006395MOV 1mg Yeast
Recombinant Pagrus major Cytochrome P450 1A1(cyp1a1) CSB-EP006395EQR 1mg E.coli
Recombinant Pagrus major Cytochrome P450 1A1(cyp1a1) CSB-YP006395EQR 1mg Yeast
Recombinant Dicentrarchus labrax Cytochrome P450 1A1(cyp1a1) CSB-EP006395DKC 1mg E.coli
Recombinant Dicentrarchus labrax Cytochrome P450 1A1(cyp1a1) CSB-YP006395DKC 1mg Yeast
Recombinant Rabbit Cytochrome P450 1A1(CYP1A1) CSB-EP006395RB 1mg E.coli
Recombinant Rabbit Cytochrome P450 1A1(CYP1A1) CSB-YP006395RB 1mg Yeast
Recombinant Human Cytochrome P450 1A1(CYP1A1) CSB-EP006395HU 1mg E.coli
Recombinant Human Cytochrome P450 1A1(CYP1A1) CSB-YP006395HU 1mg Yeast
Recombinant Mouse Cytochrome P450 1A1(Cyp1a1) CSB-EP006395MO 1mg E.coli
Recombinant Mouse Cytochrome P450 1A1(Cyp1a1) CSB-YP006395MO 1mg Yeast
Recombinant Sheep Cytochrome P450 1A1(CYP1A1) CSB-EP006395SH 1mg E.coli
Recombinant Sheep Cytochrome P450 1A1(CYP1A1) CSB-YP006395SH 1mg Yeast
Recombinant Dog Cytochrome P450 1A1(CYP1A1) CSB-EP006395DO 1mg E.coli
Recombinant Dog Cytochrome P450 1A1(CYP1A1) CSB-YP006395DO 1mg Yeast
Recombinant Guinea pig Cytochrome P450 1A1(CYP1A1) CSB-EP006395GU 1mg E.coli
Recombinant Guinea pig Cytochrome P450 1A1(CYP1A1) CSB-YP006395GU 1mg Yeast
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询