供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-EL001558RA |
CAS号: | CSB-EL001558RA |
产品名称: 大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
英文名称: Rat Delta-aminolevulinic acid dehydratase,ALAD ELISA kit
货号: CSB-EL001558RA
规格: 96T
种属: Rat
待测物名称: aminolevulinate, delta-, dehydratase
缩写: ALAD
蛋白功能1: Biosynthesis/Metabolism
蛋白功能3: Heme biosynthesis
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
用途: For research use only. Not for diagnostic use.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALAD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALAD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALAD bound in the initial step. The color development is stopped and the intensity of the color is measured.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
特异性: This assay has high sensitivity and excellent specificity for detection of Rat ALAD. No significant cross-reactivity or interference between Rat ALAD and analogues was observed.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ALAD concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
大鼠d氨基乙酰丙酸脱水酶(ALAD)ELISA kit
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蛋白
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Recombinant Aquifex aeolicus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558DNV 1mg E.coli
Recombinant Aquifex aeolicus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558DNV 1mg Yeast
Recombinant Chlamydia trachomatis Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558DSB 1mg E.coli
Recombinant Chlamydia trachomatis Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558DSB 1mg Yeast
Recombinant Archaeoglobus fulgidus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558DOC 1mg E.coli
Recombinant Archaeoglobus fulgidus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558DOC 1mg Yeast
Recombinant Candida glabrata Delta-aminolevulinic acid dehydratase(HEM2) CSB-EP001558CZI 1mg E.coli
Recombinant Candida glabrata Delta-aminolevulinic acid dehydratase(HEM2) CSB-YP001558CZI 1mg Yeast
Recombinant Methanothermobacter thermautotrophicus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558MSR 1mg E.coli
Recombinant Methanothermobacter thermautotrophicus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558MSR 1mg Yeast
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558MVZ 1mg E.coli
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558MVZ 1mg Yeast
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558SZB 1mg E.coli
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558SZB 1mg Yeast
Recombinant Escherichia coli Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558ENV 1mg E.coli
Recombinant Escherichia coli Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558ENV 1mg Yeast
Recombinant Saccharomyces cerevisiae Delta-aminolevulinic acid dehydratase(HEM2) CSB-EP001558SVG 1mg E.coli
Recombinant Saccharomyces cerevisiae Delta-aminolevulinic acid dehydratase(HEM2) CSB-YP001558SVG 1mg Yeast
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558SKX 1mg E.coli
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558SKX 1mg Yeast
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558SUL 1mg E.coli
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558SUL 1mg Yeast
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558SKY 1mg E.coli
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558SKY 1mg Yeast
Recombinant Streptomyces coelicolor Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558FOB 1mg E.coli
Recombinant Streptomyces coelicolor Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558FOB 1mg Yeast
Recombinant Helicobacter pylori Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558HUV 1mg E.coli
Recombinant Helicobacter pylori Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558HUV 1mg Yeast
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558MVN 1mg E.coli
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558MVN 1mg Yeast
Recombinant Bacillus subtilis Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558BRJ 1mg E.coli
Recombinant Bacillus subtilis Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558BRJ 1mg Yeast
Recombinant Rhodobacter capsulatus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558RLC 1mg E.coli
Recombinant Rhodobacter capsulatus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558RLC 1mg Yeast
Recombinant Bradyrhizobium japonicum Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558BVW 1mg E.coli
Recombinant Bradyrhizobium japonicum Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558BVW 1mg Yeast
Recombinant Synechococcus elongatus Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558FPY 1mg E.coli
Recombinant Synechococcus elongatus Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558FPY 1mg Yeast
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558FKZ 1mg E.coli
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558FKZ 1mg Yeast
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558MFG 1mg E.coli
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558MFG 1mg Yeast
Recombinant Schizosaccharomyces pombe Delta-aminolevulinic acid dehydratase(hem2) CSB-EP001558SXV 1mg E.coli
Recombinant Schizosaccharomyces pombe Delta-aminolevulinic acid dehydratase(hem2) CSB-YP001558SXV 1mg Yeast
Recombinant Synechocystis sp. Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558SSQ 1mg E.coli
Recombinant Synechocystis sp. Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558SSQ 1mg Yeast
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-EP001558EYU 1mg E.coli
Recombinant Delta-aminolevulinic acid dehydratase(hemB) CSB-YP001558EYU 1mg Yeast
Recombinant Rat Delta-aminolevulinic acid dehydratase(Alad) CSB-EP001558RA 1mg E.coli
Recombinant Rat Delta-aminolevulinic acid dehydratase(Alad) CSB-YP001558RA 1mg Yeast
Recombinant Mouse Delta-aminolevulinic acid dehydratase(Alad) CSB-EP001558MO 1mg E.coli
Recombinant Mouse Delta-aminolevulinic acid dehydratase(Alad) CSB-YP001558MO 1mg Yeast
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Recombinant Human Delta-aminolevulinic acid dehydratase(ALAD) CSB-YP001558HU 1mg Yeast
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