重组人丝裂原活化激酶1(Human MEK1)|1µg|PKA-256-上海希美化学有限公司

产品详情

MEK1 Human
Mitogen Activated Kinase Kinase 1 Human Recombinant
Catalog No. PKA-256

Synonyms:
MEK1, MKK1, MAPKK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAPK/ERK kinase 1, PRKMK1.

Introduction:
MAP2K1 is a member of the dual specificity protein kinase family, which plays a role as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, are recognized as extracellular signal-regulated kinases (ERKs), that act as an integration position for multiple biochemical signals. MEK1 is located upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As a key player of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. MAP2K1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a thr-glu-tyr sequence located in map kinases. MEK1 activates erk1 and erk2 map kinases.

Description:
MAP2K1 active Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain containing amino acids 2-393 having a molecular mass of 47 kDa. MAP2K1 is fused to a polyhistidine tag and is purified by proprietary chromatographic techniques.

Source:
Sf9 Insect Cells

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
MEK1 is supplied at a concentration of 0.09mg/ml in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35.

Biological Activity:
~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30°C in a reaction containing 100µM ATP.
Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3β. Kinase activity may vary depending on the substrate and reaction conditions.
The optimal concentration should be determined for each specific application.

Storage Conditions:
Store at 4°C if entire vial will be used within 1-2 weeks.
Store frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 90% as determined by SDS-PAGE.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

重组人丝裂原活化激酶1(Human MEK1)由上海希美化学有限公司为您提供，货号PKA-256，规格：1µg，CAS号：，如您想了解更多关于重组人丝裂原活化激酶1(Human MEK1)价格、重组人丝裂原活化激酶1(Human MEK1)结构式、批发、用途等信息，欢迎咨询。除供应重组人丝裂原活化激酶1(Human MEK1)外，还可为您提供人丝裂原激活的蛋白激酶/MAP激酶(MAPK)ELISA Kit 、人丝裂原活化蛋白激酶激酶6(MKK6)ELISA Kit等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

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企业名称: 上海希美化学有限公司
企业类型:
信用代码: 310228001203103
成立日期: 2009年05月31日
注册资本: 10
经营范围: 化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】

供货周期：	一周
品牌：	Prospect Biosystems
规格：	1µg
货号：	PKA-256
CAS号：