供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-E13399r |
CAS号: | CSB-E13399r |
产品名称: 大鼠环加氧酶2(COX-2)ELISA Kit
英文名称: Rat cyclooxygenase-2,COX-2 ELISA Kit
别名: COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
货号: CSB-E13399r
规格: 96T
种属: Rat
待测物名称: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
缩写: PTGS2
样本类型: serum, plasma, tissue homogenates
检测范围: 1.56 ng/ml-100 ng/ml
灵敏度: 0.39ng/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
大鼠环加氧酶2(COX-2)ELISA Kit
用途: For research use only. Not for diagnostic use.
大鼠环加氧酶2(COX-2)ELISA Kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTGS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTGS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGS2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
大鼠环加氧酶2(COX-2)ELISA Kit
特异性: This assay has high sensitivity and excellent specificity for detection of Rat PTGS2. No significant cross-reactivity or interference between Rat PTGS2 and analogues was observed.
大鼠环加氧酶2(COX-2)ELISA Kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
大鼠环加氧酶2(COX-2)ELISA Kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
大鼠环加氧酶2(COX-2)ELISA Kit
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
大鼠环加氧酶2(COX-2)ELISA Kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PTGS2 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
大鼠环加氧酶2(COX-2)ELISA Kit
产品名称 英文名称 货号 规格
人环加氧酶2(COX-2)ELISA Kit Human cyclooxygenase-2,COX-2 ELISA Kit CSB-E10103h 96T
鱼前列腺素G/H合酶2(PTGS2)ELISA kit Fish Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986FI 96T
小鼠环加氧酶2(COX-2)ELISA Kit Mouse cyclooxygenase-2,COX-2 ELISA Kit CSB-E12910m 96T
山羊前列腺素G/H合酶2(PTGS2)ELISA kit Goat Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986GO 96T
大鼠环加氧酶2(COX-2)ELISA Kit
猪前列腺素G/H合酶2(PTGS2) ELISA kit Pig prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986PI 96T
Sheep Prostaglandin G/H synthase 2(PTGS2) ELISA kit Sheep Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986SH 96T
Rabbit Prostaglandin G/H synthase 2(PTGS2) ELISA kit Rabbit Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986RB 96T
Horse Prostaglandin G/H synthase 2(PTGS2) ELISA kit Horse Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986HO 96T
大鼠环加氧酶2(COX-2)ELISA Kit
Guinea pig Prostaglandin G/H synthase 2(PTGS2) ELISA kit Guinea pig Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986GU 96T
Chicken Prostaglandin G/H synthase 2(PTGS2) ELISA kit Chicken Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986CH 96T
Bovine Prostaglandin G/H synthase 2(PTGS2) ELISA kit Bovine Prostaglandin G/H synthase 2(PTGS2) ELISA kit CSB-EL018986BO 96T
大鼠环加氧酶2(COX-2)ELISA Kit
抗体
产品名称 货号 规格
Rabbit anti-human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) polyclonal Antibody CSB-PA018986GA01HU 150ul
大鼠环加氧酶2(COX-2)ELISA Kit
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Rat Prostaglandin G/H synthase 2(Ptgs2) 1mg
Recombinant Mustela vison Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Bovine Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Horse Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Rabbit Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Chicken Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Human Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Guinea pig Prostaglandin G/H synthase 2(PTGS2) 1mg
Recombinant Sheep Prostaglandin G/H synthase 2(PTGS2) 1mg
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