供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-E09729r |
CAS号: | CSB-E09729r |
产品名称: 大鼠硫氧化还原蛋白(Trx)ELISA Kit
英文名称: Rat Thioredoxin,Trx ELISA Kit
货号: CSB-E09729r
规格: 96T
种属: Rat
待测物名称: thioredoxin
缩写: TXN
蛋白功能1: Transport
蛋白功能2: Anion transport
蛋白功能3: Electron transport
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
大鼠硫氧化还原蛋白(Trx)ELISA Kit
用途: For research use only. Not for diagnostic use.
大鼠硫氧化还原蛋白(Trx)ELISA Kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXN bound in the initial step. The color development is stopped and the intensity of the color is measured.
大鼠硫氧化还原蛋白(Trx)ELISA Kit
特异性: This assay has high sensitivity and excellent specificity for detection of Rat TXN. No significant cross-reactivity or interference between Rat TXN and analogues was observed.
大鼠硫氧化还原蛋白(Trx)ELISA Kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
大鼠硫氧化还原蛋白(Trx)ELISA Kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
大鼠硫氧化还原蛋白(Trx)ELISA Kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TXN concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
大鼠硫氧化还原蛋白(Trx)ELISA Kit
产品名称 英文名称 货号 规格
小鼠硫氧化还原蛋白(Trx)ELISA Kit Mouse Thioredoxin,Trx ELISA Kit CSB-E09730m 96T
Sheep Thioredoxin(TXN) ELISA kit Sheep Thioredoxin(TXN) ELISA kit CSB-EL025365SH 96T
Rabbit Thioredoxin(TXN) ELISA kit Rabbit Thioredoxin(TXN) ELISA kit CSB-EL025365RB 96T
Pig Thioredoxin(TXN) ELISA kit Pig Thioredoxin(TXN) ELISA kit CSB-EL025365PI 96T
Monkey Thioredoxin(TXN) ELISA kit Monkey Thioredoxin(TXN) ELISA kit CSB-EL025365RH 96T
人硫氧化还原蛋白(Trx)ELISA Kit Human Thioredoxin,Trx ELISA Kit CSB-E09728h 96T
Horse Thioredoxin(TXN) ELISA kit Horse Thioredoxin(TXN) ELISA kit CSB-EL025365HO 96T
Dog Thioredoxin(TXN) ELISA kit Dog Thioredoxin(TXN) ELISA kit CSB-EL025365DO 96T
Chicken Thioredoxin(TXN) ELISA kit Chicken Thioredoxin(TXN) ELISA kit CSB-EL025365CH 96T
Bovine Thioredoxin(TXN) ELISA kit Bovine Thioredoxin(TXN) ELISA kit CSB-EL025365BO 96T
大鼠硫氧化还原蛋白(Trx)ELISA Kit
抗体
产品名称 货号 规格
Rabbit anti-human thioredoxin polyclonal Antibody CSB-PA025365GA01HU 150ul
大鼠硫氧化还原蛋白(Trx)ELISA Kit
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Staphylococcus epidermidis Thioredoxin(trxA) 1mg
Recombinant Staphylococcus saprophyticus subsp. saprophyticus Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Rickettsia bellii Thioredoxin(trxA) 1mg
Recombinant Rickettsia prowazekii Thioredoxin(trxA) 1mg
Recombinant Chlamydia pneumoniae Thioredoxin(trxA) 1mg
Recombinant Pseudomonas aeruginosa Thioredoxin(trxA) 1mg
Recombinant Coprinus comatus Thioredoxin 1mg
Recombinant Mycoplasma gallisepticum Thioredoxin(trxA) 1mg
Recombinant Chlamydia muridarum Thioredoxin(trxA) 1mg
Recombinant Ictalurus punctatus Thioredoxin(txn) 1mg
Recombinant Pasteurella multocida Thioredoxin(trxA) 1mg
Recombinant Ophiophagus hannah Thioredoxin(TXN) 1mg
Recombinant Callithrix jacchus Thioredoxin(TXN) 1mg
Recombinant Mycoplasma pulmonis Thioredoxin(trxA) 1mg
Recombinant Rickettsia conorii Thioredoxin(trxA) 1mg
Recombinant Staphylococcus epidermidis Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Chloroflexus aurantiacus Thioredoxin(trxA) 1mg
Recombinant Plasmodium falciparum Thioredoxin(PF14_0545) 1mg
Recombinant Rickettsia typhi Thioredoxin(trxA) 1mg
Recombinant Pongo abelii Thioredoxin(TXN) 1mg
Recombinant Methanocaldococcus jannaschii Thioredoxin(trx) 1mg
Recombinant Rickettsia felis Thioredoxin(trxA) 1mg
Recombinant Bacillus subtilis Glycine oxidase(thiO) 1mg
Recombinant Rhizobium etli Putative thiamine biosynthesis oxidoreductase thiO(thiO) 1mg
Recombinant Schizosaccharomyces pombe Thioredoxin-1(trx1) 1mg
Recombinant Salmonella typhimurium Thioredoxin-1(trxA) 1mg
Recombinant Thioredoxin-1(trxA) 1mg
Recombinant Anabaena sp. Thioredoxin-1(trxA) 1mg
Recombinant Thioredoxin-1(trxA) 1mg
Recombinant Thioredoxin-1(trxA) 1mg
Recombinant Thioredoxin-1(trxA) 1mg
Recombinant Escherichia coli Thioredoxin-1(trxA) 1mg
Recombinant Nostoc sp. Thioredoxin-1(trxA) 1mg
Recombinant Dictyostelium discoideum Thioredoxin-1(trxA) 1mg
Recombinant Arabidopsis thaliana Thioredoxin H1(TRX1) 1mg
Recombinant Saccharomyces cerevisiae Thioredoxin-1(TRX1) 1mg
Recombinant Synechococcus elongatus Thioredoxin-1(trxA) 1mg
Recombinant Mycobacterium tuberculosis Thioredoxin(trxA) 1mg
Recombinant Listeria innocua serovar 6a Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Chromatium vinosum Thioredoxin(trxA) 1mg
Recombinant Mycobacterium bovis Thioredoxin(trxA) 1mg
Recombinant Listeria monocytogenes serovar 1/2a Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Rhodobacter sphaeroides Thioredoxin(trxA) 1mg
Recombinant Helicobacter pylori Thioredoxin(trxA) 1mg
Recombinant Helicobacter pylori Thioredoxin(trxA) 1mg
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Thioredoxin(trxA) 1mg
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Thioredoxin(trxA) 1mg
Recombinant Chlamydophila caviae Thioredoxin(trxA) 1mg
Recombinant Streptomyces coelicolor Thioredoxin(trxA) 1mg
Recombinant Synechocystis sp. Thioredoxin(trxA) 1mg
Recombinant Thiobacillus ferrooxidans Thioredoxin(trxA) 1mg
Recombinant Porphyra yezoensis Thioredoxin(trxA) 1mg
Recombinant Mycoplasma genitalium Thioredoxin(trxA) 1mg
Recombinant Porphyra purpurea Thioredoxin(trxA) 1mg
Recombinant Griffithsia pacifica Thioredoxin(trxA) 1mg
Recombinant Haemophilus influenzae Thioredoxin(trxA) 1mg
Recombinant Streptomyces aureofaciens Thioredoxin(trxA) 1mg
Recombinant Emericella nidulans Thioredoxin(TRX1) 1mg
Recombinant Neurospora crassa Thioredoxin(trx) 1mg
Recombinant Cyanidium caldarium Thioredoxin(trxA) 1mg
Recombinant Penicillium chrysogenum Thioredoxin(TRXA) 1mg
Recombinant Eubacterium acidaminophilum Thioredoxin(trxA) 1mg
Recombinant Clostridium litorale Thioredoxin(trxA) 1mg
Recombinant Bacillus subtilis Thioredoxin(trxA) 1mg
Recombinant Rhodospirillum rubrum Thioredoxin(trxA) 1mg
Recombinant Rat Thioredoxin(Txn) 1mg
Recombinant Chlorobium limicola f.sp. thiosulfatophilum Thioredoxin(trxA) 1mg
Recombinant Thiocapsa roseopersicina Thioredoxin(trxA) 1mg
Recombinant Staphylococcus aureus Thioredoxin(trxA) 1mg
Recombinant Mycoplasma pneumoniae Thioredoxin(trxA) 1mg
Recombinant Alicyclobacillus acidocaldarius Thioredoxin(trxA) 1mg
Recombinant Treponema pallidum Thioredoxin(trxA) 1mg
Recombinant Geodia cydonium Thioredoxin(THIO) 1mg
Recombinant Chlamydia trachomatis Thioredoxin(trxA) 1mg
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Thioredoxin(trxA) 1mg
Recombinant Borrelia burgdorferi Thioredoxin(trxA) 1mg
Recombinant Mycobacterium smegmatis Thioredoxin(trxA) 1mg
Recombinant Echinococcus granulosus Thioredoxin(TRX) 1mg
Recombinant Cyanidioschyzon merolae Thioredoxin(trxA) 1mg
Recombinant Bovine Thioredoxin(TXN) 1mg
Recombinant Horse Thioredoxin(TXN) 1mg
Recombinant Rabbit Thioredoxin(TXN) 1mg
Recombinant Chicken Thioredoxin(TXN) 1mg
Recombinant Sheep Thioredoxin(TXN) 1mg
Recombinant Macaca mulatta (Rhesus macaque) Thioredoxin(TXN) 1mg
Recombinant Human Thioredoxin(TXN) 1mg
Recombinant Mouse Thioredoxin(Txn) 1mg
Recombinant Dog Thioredoxin(TXN) 1mg
Recombinant Pig Thioredoxin(TXN) 1mg
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