供货周期: | 一周 |
品牌: | |
规格: | 96T |
货号: | CSB-E04990h |
CAS号: | CSB-E04990h |
产品名称: 人甲状腺球蛋白(TG)ELISA Kit
英文名称: Human Thyroglobulin,TG ELISA Kit
别名: AITD3, TGN
货号: CSB-E04990h
规格: 96T
种属: Human
待测物名称: thyroglobulin
缩写: TG
蛋白功能1: Biosynthesis/Metabolism
蛋白功能3: Thyroid hormones biosynthesis
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TG bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human TG. No significant cross-reactivity or interference between Human TG and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
相关产品:
产品名称 英文名称 货号 规格
人甘油三酯(TG)ELISA Kit Human Triglyceride,TG ELISA Kit CSB-E14266h 96T
大鼠甘油三酯(TG)ELISA Kit Rat Triglyceride,TG ELISA Kit CSB-E11705r 96T
小鼠甘油三酯(TG)ELISA Kit Mouse Triglyceride,TG ELISA Kit CSB-E14268m 96T
Dog Thyroglobulin(TG) ELISA kit Dog Thyroglobulin(TG) ELISA kit CSB-EL023442DO 96T
大鼠甲状腺球蛋白(TG)ELISA Kit Rat Thyroglobulin,TG ELISA Kit CSB-E08240r 96T
小鼠甲状腺球蛋白(TG)ELISA Kit Mouse Thyroglobulin,TG ELISA Kit CSB-E08241m 96T
Bovine Thyroglobulin(TG) ELISA kit Bovine Thyroglobulin(TG) ELISA kit CSB-EL023442BO 96T
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Mouse Thyroglobulin(Tg) CSB-EP023442MO 1mg E.coli
Recombinant Mouse Thyroglobulin(Tg) CSB-YP023442MO 1mg Yeast
Recombinant Human Thyroglobulin(TG) CSB-EP023442HU 1mg E.coli
Recombinant Human Thyroglobulin(TG) CSB-YP023442HU 1mg Yeast
Recombinant Bovine Thyroglobulin(TG) CSB-EP023442BO 1mg E.coli
Recombinant Bovine Thyroglobulin(TG) CSB-YP023442BO 1mg Yeast
Recombinant Rat Thyroglobulin(Tg) CSB-EP023442RA 1mg E.coli
Recombinant Rat Thyroglobulin(Tg) CSB-YP023442RA 1mg Yeast
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
Mouse Glutamate receptor, ionotropic kainate 1(GRIK1)ELISA kit
3600
Mouse Glutamate receptor-interacting protein 1(GRIP1)ELISA kit
3600
Mouse Glutaminase liver isoform, mitochondrial(GLS2)ELISA kit
3600
Mouse GPRC6A ELISA kit
3600
Mouse G-rich sequence factor 1(GRSF1)ELISA kit
3600
Mouse GRIP and coiled-coil domain-containing protein 1(GCC1)ELISA kit
3600
Mouse GRIP and coiled-coil domain-containing protein 2(GCC2)ELISA kit
3600
Mouse GRIP1-associated protein 1(GRIPAP1)ELISA kit
3600
详细地址
关注
拨打电话
留言咨询