供货周期: | 一周 |
品牌: | |
规格: | 10μg/50μg/1mg |
货号: | CYT-433 |
CAS号: |
Prospec (蛋白质专家) 是一家领先的生物科技公司,有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子,生长因子,趋化因子,激素,酶,病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利,所以Prospec以生产成本低,生产效率高,产品纯度高,有竞争力的价格,使Prospec产品在市场上立于不败之地(占有很大优势)。
Prospec为满足顾客的需要,还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构,政府研究部门,生物科技和药物公司以及医院和参照实
Acrp30 Human, His
Adiponectin Human Recombinant, His tag
Catalog No. CYT-433
Synonyms:
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Introduction:
Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
Description:
The Acrp30 Human is created as a recombinant protein with N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the Acrp30 Human and 12 additional amino acid residues - HisTag (underlined).
MRGSHHHHHHGSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD TN
Specificity:
The amino acid sequence of the Acrp30 Human Recombinant is 100% homologous to the amino acid sequence of the Adiponectin human serum.
Purification Method:
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Source:
Escherichia Coli.
Purity:
Acrp30 Human purity is greater than 95% as determined by SDS-PAGE.
Formulation:
Acrp30 Human is a sterile filtered powder, lyophilized from 0.5 mg/ml in 0.02M Tris buffer pH7.5, 0.15M NaCl.
Solubility:
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet of Acrp30 Human dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.
Stability:
Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized Acrp30 Human remains stable until the expiry date when stored at -20°C.
Quality Control Test:
BCA - to determine content of the protein.
SDS PAGE - to determine purity of the protein.
Applications:
Western blotting.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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