Recombinant Human APO-D(重组人载脂蛋白-D)

Prospec (蛋白质专家) 是一家领先的生物科技公司，有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子，生长因子，趋化因子，激素，酶，病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利，所以Prospec以生产成本低，生产效率高，产品纯度高，有竞争力的价格，使Prospec产品在市场上立于不败之地（占有很大优势）。
Prospec为满足顾客的需要，还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构，政府研究部门，生物科技和药物公司以及医院和参照实

Apo D Human
Apoliportein-D Human Recombinant
Catalog No. CYT-547 
Synonyms:
Apolipoprotein D, Apo-D, ApoD.


Introduction:
Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid.
Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer's disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.

Description:
Apoliprotein-D Human Recombinant His Tag fusion protein at C-terminus (7 highlighted a.a.) produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa.
The protein a.a.sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.
The Following gene modifications were made:
Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apoliprotein-D to enhance the protein’s solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchnages which facilitate its genetic manipulation. The Apoliprotein-D is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.





Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH7.5.

Solubility:
Add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely.

Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Purity:
Greater than 95% as determined by SDS-PAGE.

Amino Acid Sequence:
FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.