供货周期: | 一周 |
品牌: | Prospect Biosystems |
规格: | 10μg/50μg/1mg |
货号: | CYT-402 |
CAS号: | CYT-402 |
Prospec (蛋白质专家) 是一家领先的生物科技公司,有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子,生长因子,趋化因子,激素,酶,病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利,所以Prospec以生产成本低,生产效率高,产品纯度高,有竞争力的价格,使Prospec产品在市场上立于不败之地(占有很大优势)。
Prospec为满足顾客的需要,还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构,政府研究部门,生物科技和药物公司以及医院和参照实
IFN g Porcine
Interferon-Gamma Porcine Recombinant
Catalog No. CYT-402
Synonyms:
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Introduction:
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Description:
Interferon-gamma Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 17140 Dalton.
The IFN-gamma is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized with no additives.
Solubility:
It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Interferon-g although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Cys-Gln-Ala.
Biological Activity:
The ED50 as determined by the amount of interferon that inhibited 50% of the cytopathic effect of vesicular stomatitis virus in MDBK cells was < 0.3 ng/ml.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.556 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFNg as a Reference Standard.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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