重组人瘦素拮抗剂(Human Leptin Quadruple Antagonist)

重组人瘦素拮抗剂(Human Leptin Quadruple Antagonist)

参考价：面议
供货周期： 一周
品牌： Prospect Biosystems
规格： 20μg/100μg/1mg
货号： CYT-353
CAS号：
上海希美化学有限公司
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Leptin qA Human
Leptin Antagonist Quadruple Mutant Human Recombinant
Catalog No. CYT-353
Description:
Leptin Quadruple Mutant Human Recombinant is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a Mw of 16 kDa, Human Leptin was mutated, resulting in L39A/D40A/F41A/I42A.
Leptin Antagonist Quadruple Mutant was purified by proprietary chromatographic techniques.

Source:
Escherichia coli.

Physical Appearance:
White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Solubility:
It is recommended to reconstitute the lyophilized Leptin Antagonist Quadruple Mutant in sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Leptin Antagonist Quadruple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln

Biological Activity:
ProSpec’s Leptin Quadruple Antagonist Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.

Protein content:
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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重组人瘦素拮抗剂(Human Leptin Quadruple Antagonist)由上海希美化学有限公司为您提供，货号CYT-353，规格：20μg/100μg/1mg，CAS号：，如您想了解更多关于重组人瘦素拮抗剂(Human Leptin Quadruple Antagonist)价格、重组人瘦素拮抗剂(Human Leptin Quadruple Antagonist)结构式、批发、用途等信息，欢迎咨询。除供应重组人瘦素拮抗剂(Human Leptin Quadruple Antagonist)外，还可为您提供重组人瘦素(Human Leptin)、重组人瘦素 His(Human Leptin His)等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。
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