重组小鼠可溶性CD40配体(Mouse soluble CD40 Ligand/TRAP)|5μg/25μg/1mg|CYT-472-上海希美化学有限公司

产品详情

sCD40 Mouse
Soluble CD-40 Ligand/TRAP Mouse Recombinant
Catalog No. CYT-472

Synonyms:
CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L.

Introduction:
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines . CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma , TNF-alpha , and IL2 .

Description:
sCD40 Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16409 Dalton.
The sCD40 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from a sterile concentrated solution (1mg/ml) with 10mM Sodium Phosphate pH=7.5.

Solubility:
It is recommended to reconstitute the lyophilized sCD40 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized sCD40 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD154 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Arg-Gly-Asp.

Biological Activity:
The ED50 as determined by its ability to induce MIP-1 alpha & TNF-alpha from mouse splenocytes was found to be 0.1 µg/ml.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.1 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of sCD40 as a Reference Standard.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

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重组小鼠可溶性CD40配体(Mouse soluble CD40 Ligand/TRAP)由上海希美化学有限公司为您提供，货号CYT-472，规格：5μg/25μg/1mg，CAS号：，如您想了解更多关于重组小鼠可溶性CD40配体(Mouse soluble CD40 Ligand/TRAP)价格、重组小鼠可溶性CD40配体(Mouse soluble CD40 Ligand/TRAP)结构式、批发、用途等信息，欢迎咨询。除供应重组小鼠可溶性CD40配体(Mouse soluble CD40 Ligand/TRAP)外，还可为您提供兔子可溶性CD40配体(sCD40L)ELISA Kit、人可溶性CD40配体(sCD40L)ELISA Kit 等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

供货周期：	一周
品牌：	Prospect Biosystems
规格：	5μg/25μg/1mg
货号：	CYT-472
CAS号：