重组β内酰胺酶(b-Lactamase)|1mg/10mg/50mg|ENZ-351-上海希美化学有限公司

产品详情

β Lactamase
Beta Lactamase Recombinant
Catalog No. ENZ-351

Synonyms:
β-Lactamase, EC 3.5.2.6, Cephalosporinase.

Introduction:
Beta-lactamase is a type of enzyme(EC3.5.2.6) produced by some bacteria that is responsible for their resistanceto beta-lactam antibioticslike penicillins, cephalosporins, cephamycinsand carbapenems. These antibioticshave a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties.

Description:
Beta-Lactamase Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa.
Beta Lactamase is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH-7.

Solubility:
It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

Unit Definition:
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 ° C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Specific Activity:
1,500IU/mg.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

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工商信息

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企业名称: 上海希美化学有限公司
企业类型:
信用代码: 310228001203103
成立日期: 2009年05月31日
注册资本: 10
经营范围: 化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】

供货周期：	一周
品牌：	Prospect Biosystems
规格：	1mg/10mg/50mg
货号：	ENZ-351
CAS号：