供货周期: | 一周 |
品牌: | Prospect Biosystems |
规格: | 500μg/2mg/75mg |
货号: | ENZ-319 |
CAS号: |
DNase Human
Deoxyribonuclease I Human Recombinant
Catalog No. ENZ-319
Synonyms:
EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1.
Introduction:
Deoxyribonuclease I Human Recombinant (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2 .
Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.
Description:
Deoxyribonuclease I Human Recombinant produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1995O396S9.
DNase is purified by proprietary chromatographic techniques.
Source:
Chinese Hamster Ovary Cells
Physical Appearance:
Sterile liquid colorless solution at a concentration of 1mg/ml.
Formulation:
Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.
Stability:
2 years when stored at 4°C, three weeks at 15°C, pH-6.3.
Specific Activity:
1000IU/1mg
Unit Defenition:
Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1µgof lambda phage DNA in 10 minutes at 37°C in50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» DNase Bovine
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询