供货周期: | 一周 |
品牌: | Prospect Biosystems |
规格: | 2μg/10μg/1mg |
货号: | ENZ-375 |
CAS号: |
DPP4 Human
Dipeptidyl-Peptidase 4 Human Recombinant
Catalog No. ENZ-375
Synonyms:
CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen.
Introduction:
DPP4 also called adenosine deaminase complexing protein-2, and T-cell activation antigen CD26 is a serine exopeptidase and complex enzyme that is expressed on the surface of most cell types. DPPIV is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP4 plays a role in t-cell activation. DPP4 is associated with intracellular signal transduction, apoptosis and involved in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1).
Description:
DPPIV Human Recombinant produced in High-5 cells is a single, glycosylated polypeptide chain containing 746 amino acids (39-766) and having a molecular mass of 86.4 kDa.
DPPIV is fused to His Tag at C-terminus and purified using conventional chromatography techniques.
Source:
High-5 cells
Physical Appearance:
Sterile Filtered colorless solution.
Formulation:
DPP4 is formulated in 20mM Tris-HCl pH-8, 100mM NaCl, 1mM EDTA and 10% glycerol.
Stability:
DPP4 although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Unit Definition:
One unit will hydrolyze 1 mmole of p-nitroaniline per minute at pH8.0 at 37°C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Biological Activity:
20 Units/mg
Assay Methods:
- Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA
- Total reaction volume: 100 ul
- Reaction temperature: 37°C
1. Add the reaction buffer to each well.
2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.
3. Add the enzyme (DPP-4) diluent to each well.
4. Incubate the 96 well plate at 37°C.
5. Read the optical density at 405 nm.
Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate
0ng DPP4= 0 OD at 405nm
20ng DPP4= 0.075 OD at 405nm
40ng DPP4= 0.125 OD at 405nm
60ng DPP4= 0.2 OD at 405nm
80ng DPP4= 0.275 OD at 405nm
100ng DPP4= 0.35 OD at 405nm
Amino Acid Sequence:
ADP-SRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENN
ILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQ
FILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIP
NNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRIT
WTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFL
AYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAG
AVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGD
HYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESS
GRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSF
YKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIE
ALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCL
SCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTL
HSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIIL
NETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKAD
TVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHA
INRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWS
YGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYT
ERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIH
GTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHG
IASSTAHQHIYTHMSHFIKQCFSLP-SGRLVPRGSHHHHHH
Purity:
Greater than 95.0% as determined by Analysis by SDS-PAGE.
On SDS-PAGE under denatured condition, apparent molecular weight of glycosylated DPP4 will migrate at approximately 90kDa
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» DPP4 Human
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询