重组二硫键氧化还原酶（DsbA）|10μg/50μg/1mg|ENZ-276-上海希美化学有限公司

产品详情

DsbA
Disulfide Oxidoreductase Recombinant
Catalog No. ENZ-276

Synonyms:
DsbA, Thiol:disulfide interchange protein dsbA.

Introduction:
DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.

Description:
Disulfide Oxidoreductase produced in E.Coli is a periplasmic protein isolated from E. coli, containing 208 amino acids having a molecular mass of 23,149 Dalton.
The DsbA is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride.

Solubility:
It is recommended to reconstitute the lyophilized DsbA in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DsbA should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids
was determined and was found to be Met-Ly-Lys-Ala-Trp.

Applications:
Suitable for use in Western Blot. If this protein is to be used for Western Blot analysis, we recommend that the material be diluted in 1X SDS-PAGE sample buffer (1). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot with D9625 at a dilution of 1:10,000 as the primary antibody and an appropriate alkaline phosphatase conjugated secondary antibody for detection.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

重组二硫键氧化还原酶（DsbA）由上海希美化学有限公司为您提供，货号ENZ-276，规格：10μg/50μg/1mg，CAS号：，如您想了解更多关于重组二硫键氧化还原酶（DsbA）价格、重组二硫键氧化还原酶（DsbA）结构式、批发、用途等信息，欢迎咨询。除供应重组二硫键氧化还原酶（DsbA）外，还可为您提供Recombinant E.Coli Thiol DsbG、Recombinant E.Coli DsbE等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

Business information

工商信息

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企业名称: 上海希美化学有限公司
企业类型:
信用代码: 310228001203103
成立日期: 2009年05月31日
注册资本: 10
经营范围: 化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】

供货周期：	一周
品牌：	Prospect Biosystems
规格：	10μg/50μg/1mg
货号：	ENZ-276
CAS号：