供货周期: | 一周 |
品牌: | Prospect Biosystems |
规格: | 1μg/2.5μg/10μg |
货号: | ENZ-317 |
CAS号: |
MMP 13 Human
Matrix Metalloproteinase-13 Human Recombinant
Catalog No. ENZ-317
Synonyms:
Collagenase 3, EC 3.4.24.-, Matrix metalloproteinase-13, MMP-13, CLG3.
Introduction:
Latent recombinant human pro-collagenase (MMP-13) also called collagenase-3 truncated from C-terminal.
Matrix Metalloproteinase-13 (MMP-13) is an enzyme that is a member of the MMP extracellular protease family. Extracellular protease enzymes, by virtue of their broad substrate specificities1, play a role in both normal and disease states of tissue proliferation. Among the targets of MMP-13 are collagen, gelatin, entactin, pro-TNF-a, and chemokine SDF-11-4.
MMP-13 is found in its latent form as a 52-56 kDa glycosylated proenzyme. Upon cleavage the 22-46 kDa5 MMP-1 becomes active in extracellular matrix remodeling.
Because of the prominent role that MMP-1 plays in cell migration and metastasis, it is an important target for inhibition screening.
Description:
Matrix Metalloproteinase-13 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27 kDa.
The Collagenase 3 is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered clear solution.
Formulation:
The protein Solution (100 µg/ml) in 0.05M Tris-HCl buffer, pH 7.6, containing 0.2M NaCl, 5mM CaCl2, 20µM ZnSO4 and 0.1% BSA and NaN3.
Stability:
MMP-13 although stable at 4°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 90% as determined by SDS-PAGE.
Biological Activity:
Activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 75-80% (1.5-1.6 nmole) of fluorogenic peptide substrate (0.1ml of 20µM solution) at 35°C for 30 minutes.
Applications:
Used as a standard for assaying MMP-13 or for screening inhibitors.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» MMP 3 Human
» MMP 3 Human, GST
» MMP 7 Human
» MMP 8 Human
» MMP 9 Human
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询