| 供货周期： | 一周 |
| 品牌： | Prospec-Tany |
| 规格： | 50IU/100IU/200IU |
| 货号： | ENZ-266 |
| CAS号： | ENZ-266 |
PPK
Polyphosphate Kinase Recombinant
Catalog No. ENZ-266
Synonyms:
Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase.
Introduction:
PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Description:
Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity.
Native MW = 83 kD (monomer)
Source:
Propionibacterium shermanii
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized from PPK solution containing 11.54 U/ml of PPK activity, 10.3 mg/ml total protein, 100mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.
Solubility:
The contents of the vial can be reconstituted in 1-10 ml deionized water.
Stability:
Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Definition of Activity Units:
The amount of Polyphosphate kinase required to convert 1 µmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.
Specific Activity:
1.12 U/mg.
Required Cofactors:
The reaction requires Mg2+.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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