重组Taq DNA聚合酶(Taq DNA)

TaqDNA
Taq DNA Polymerase Recombinant
Catalog No. ENZ-308

Synonyms:
DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1.

Description:
Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74ºC and exhibits a half-life of 40 minutes at 95ºC. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

Source:
Recombinant e.coli contains Thermus aquaticus polymerase gene.

Formulation:
Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74ºC. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25ºC), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10μg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.

10X Reaction Buffer with MgCl2:
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.

10X Reaction Buffer without MgCl2:
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100.Include: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.

Features:
Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions.

Specify Your Own Reaction Conditions:
Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2.

Rely on a Performance-Tested Enzyme:
Our PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.

Applications:
PCR, 3´ A-tailing of blunt ends, compatible with Vectors.

Storage Conditions:
Stable for 5 days at 10°C, for longer period of time store at -20°C.

Storage Buffer:
Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme.
50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.

Quality Control Tests:
PCR, activity, SDS-PAGE/purity, endonuclease/nickase.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.