| 供货周期： | 一周 |
| 品牌： | Prospect Biosystems |
| 规格： | 2000U/10000U/20000U |
| 货号： | ENZ-352 |
| CAS号： |
UNG
Uracil DNA Glycosilase
Catalog No. ENZ-352
Synonyms:
Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.
Description:
E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).
Source:
Escherichia Coli strain that carries the UNG gene from E.coli
Physical Appearance:
Sterile Filtered colorless solution.
Formulation:
10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
Stability:
Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Applications:
Glycosilase mediated single nucleotide polymorphism detection (GMPD).
Site-directed mutagenesis.
As a probe for protein-DNA interaction sudies.
Rapid and efficient cloning of PCR products.
Elimination carry-over contamination in PCR.
Specific Activity:
The Specific Activity was found to be 5U/μl.
Unit Definition:
1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60pmol of Uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50µl in 30 minutes at 37°C in 1X Uracil DNA Glycosilase Reaction Buffer with 1 Unit of Uracil DNA Glycosylase and 0.2µg [3H]-Uracil DNA (104- 105 cpm/µg)
10X UNG Reaction Buffer:
200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.
Reaction Conditions:
1X UNG Reaction Buffer, incubate at 37°C.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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