| 供货周期： | 一周 |
| 品牌： | Prospect Biosystems |
| 规格： | 1mg/5mg/50mg |
| 货号： | ENZ-277 |
| CAS号： |
Urease
Urease Recombinant
Catalog No. ENZ-277
Description:
Urease (EC3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows:
(NH2)2CO+ H2O → CO2+ 2NH3
In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants.
Characteristics: Active sitemetal: nickel(II); Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence); Optimum pH: 7.4; Optimum Temperature: 60 degrees Celsius; Enzymatic specificity: urea and hydroxyurea; Inhibitors: heavy metals.
The multi-subunit enzyme usually has a 3:3 (alpha:beta) stoichiometry with a 2-fold symmetric structure (note that the image above gives the structure of the asymmetric unit, one third of the true biological assembly). An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits (α12β12). This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise gastric acid. The presence of urease is used in the diagnosis of Helicobacter species.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Lyophilized Powder.
Formulation:
Each mg of protein contains 345µg Potassium Phosphate and 25µg EDTA Na2.
Solubility:
It is recommended to reconstitute the lyophilized Urease in sterile 18MΩ-cm H2O.
Stability:
Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
The specific activity was found to be 150 IU/mg.
Unit Definition:
One Unit oxidizes one micromole of NADH per minute at 25°C, at pH 7.6.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit（3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒）
3-D水凝胶PEG-Link（聚乙二醇交联剂）（3D细胞培养 PEG 交联物（非降解））
3-D Life CD-Link（3D细胞培养 CD 交联物（可细胞降解））
3-D Life Maleimide-Dextran Set（3D 细胞培养葡聚糖马来酰亚胺聚合物）
3D 细胞培养RGD多肽（粘附剂）3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
工商信息
信息已认证
联系方式
详细地址
关注
拨打电话
留言咨询
