Human AKT1|2μg/10μg/1mg|PKA-305-上海希美化学有限公司

产品详情

AKT1 Human
AKT1 Human Recombinant
Catalog No. PKA-305

Synonyms:
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, C-AKT, AKT, PKB, RAC, PRKBA, MGC99656, RAC-ALPHA.

Introduction:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Description:
AKT1 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 481 amino acids and having a molecular mass of 60 KD. This protein is the full-length form of the protein with a N-terminal His-tag.
AKT1 is purified by proprietary chromatographic techniques.

Source:
Baculovirus, SF9 insect cells.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
AKT1 (0.1mg/ml) is supplied in 50mM Tris pH 7.5, 0.15M NaCl, 0.27M sucrose,10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free.

Unit Definition:
250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30°C with a final ATP concentration of 100µM. The optimal concentration should be determined for each specific application.
Please note:Kinase activity may vary depending on the substrate and reaction conditions.

Storage Conditions:
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C- -80°C for longer periods of time. Avoid multiple freeze-thaw cycles. Purity: Greater than 95% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Human AKT1由上海希美化学有限公司为您提供，货号PKA-305，规格：2μg/10μg/1mg，CAS号：，如您想了解更多关于Human AKT1价格、Human AKT1结构式、批发、用途等信息，欢迎咨询。除供应Human AKT1外，还可为您提供人体血红蛋白A1c(Human Hemoglobin A1c/HbA1c)、重组人乙酰辅酶A乙酰转移酶2(Human ACAT2)等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。

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工商信息

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企业名称: 上海希美化学有限公司
企业类型:
信用代码: 310228001203103
成立日期: 2009年05月31日
注册资本: 10
经营范围: 化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】

供货周期：	一周
品牌：	Prospect Biosystems
规格：	2μg/10μg/1mg
货号：	PKA-305
CAS号：