Recombinant C-JUN

Recombinant C-JUN

参考价:面议
供货周期: 一周
品牌: Prospect Biosystems
规格: 2μg/10μg/1mg
货号: PKA-323
CAS号:
上海希美化学有限公司
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C-JUN Human
C-JUN Human Recombinant
Catalog No. PKA-323

Synonyms:
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, c-Jun.

Introduction:
C-JUN is a gene which, in combination with c-Fos, forms the AP-1early response transcription factor. It is activated by the JNKpathway.
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Description:
C-JUN amino acids 1-81 produced in E.coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa.
C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
C-JUN is supplied as lyophilized powder containing no additives.

Solubility:
It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml.

Storage Conditions:
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.


Biological Activity:
C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.

Please note:
Kinase activity may vary depending on the substrate and reaction conditions.

Purity:
Greater than 95% as determined by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Recombinant C-JUN由上海希美化学有限公司为您提供,货号PKA-323,规格:2μg/10μg/1mg,CAS号:,如您想了解更多关于Recombinant C-JUN价格、Recombinant C-JUN结构式、批发、用途等信息,欢迎咨询。除供应Recombinant C-JUN外,还可为您提供大鼠c-fos ELISA Kit、大鼠c-jun ELISA Kit 等试剂,公司有专业的客户服务团队,是您值得信赖的合作伙伴,上海希美化学客户服务电话,售前、售后均可联系。
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