PKACa2/RIIa2|1μg/3μg/100μg|PKA-205-上海希美化学有限公司

PKACa2/RIIa2

参考价：面议

供货周期：

一周

品牌：

Prospect Biosystems

规格：

1μg/3μg/100μg

货号：

PKA-205

CAS号：

产品详情

PKACa2-RIIa2
Protein Kinase A holoenzyme type II alpha Recombinant
Catalog No. PKA-205

Synonyms:
Protein Kinase A holoenzyme type II alpha, PKACa2-RIIa2.

Introduction:
The protein kinase A holoenzyme is a heterotetramer composed of two types of subunits: Catalytic and Regulatory. The Catalyticsubunit contains the enzyme's active site. It also contains a domain that binds ATP and a domain that binds the regulatory subunit. The Regulatory subunit consists oftwo molecules which bind one another in an anti-parallel orientation to form a homodimer; for type I subunits- this binding is covalent via disulfide bonds. This subunit also has has two domains that bind cyclic AMP, a domain that interacts with a catalytic subunit, and an "auto-inhibitory" domain that serves as a substrate or pseudosubstrate for the catalytic subunit. Regulatory subunits may also have biologic activity distinct from their role in modulating catalytic subunit activity. Regulatory subunits exist in two major forms, RI and RII, with each form having two subtypes designated alpha and beta. Each of the four isotypes of the regulatory subunit is encoded by a different gene. In addition, three isotypes of the catalytic subunit have been identified (alpha, beta and gamma). The different isotypes tend to have different distributions within cells and among tissues. Type I enzymes inhabit cytoplasmic, soluble fractions of the cell, whereas type II enzymes tend to associate with cellular membranes.

Description:
Inactive holoenzyme consisting of one dimeric regulatory subunit type II alpha and two monomeric catalytic subunits (cAMP-free).
Protein Kinase A is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered clear solution.

Formulation:
PKA holoenzyme type-II alpha is supplied in 50% glycerol.

Stability:
PKA should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C.
Avoid multiple freeze-thaw cycles.

Purity:
Greater than 95% as determined by SDS-PAGE.

Biological Activity:
Holoenzyme can be activated by adding the second messenger cAMP (Activation constant about 100nM) releasing two monomeric catalytic subunits.

Applications:
The product is suitable for analysing PKA type II agonists (cAMP analogs) or antagonists.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Business information

工商信息

信息已认证

企业名称: 上海希美化学有限公司
企业类型:
信用代码: 310228001203103
成立日期: 2009年05月31日
注册资本: 10
经营范围: 化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】