Recombinant EAST6

ESAT6
Early Secretory Target Mycobacterium Tuberculosis Recombinant
Catalog No. PRO-291

Synonyms:
Early Secretory Target Mycobacterium Tuberculosis, ESAT-6.

Introduction:
Mycobacterium antigen ESAT-6 has been isolated from low molecular weight fractions of the shot-term-culture filtrate (ST-CF) and it can easily be detected in tuberculosis patients.
The export of ESAT-6 which is a potent T-cell antigen, and related proteins requires a dedicated secretory apparatus that is encoded by a group of genes, several of which also code for proteins that are recognized strongly by T cells. The ESAT-6 systems can consequently be considered as immunogenicity islands and there is mounting evidence that the equivalent genes are subject to selective pressure imposed by the immune system of the host.
This antigen includes many epitopes detectable in the serum of most patients with tuberculosis (more than 90%). By the attempts to obtain the vaccine on the basis of ESAT-6 it was demonstrated that the optimization of adjuvant is very important when using the combination of dioctadecylammonium bromide and monophosphoryllipide.
Recently it was shown that ESAT-6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. The main topic in ESAT-6 using is in antibody production and in test-systems for tuberculosis elaboration.

Patent Rights:
The sale and/or commercial use of Recombinant ESAT-6 is prohibited in the United States of America (U.S.A), Canada, Great Britain, Denmark, France, Greece, Italy, Germany, Spain, Ireland, Portugal, New Zealand and Australia.

Description:
ESAT-6 Recombinant produced in e.coli is a single, non-glycosylated, polypeptide having a total molecular mass of 11261.35 Dalton.
The ESAT-6 contains 6 additional amino acid residues - His-Tag.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein (1mg/ml) was lyophilized after from a sterile solution containing 50mM Tris-HCl, pH 8.0, 1mM EDTA and 1mM DTT.

Solubility:
It is recommended to reconstitute the lyophilized ESAT-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized ESAT-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ESAT-6 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino Acid Composition:
AEQQWNFAGIEAAASAIQGNVTS
IHSLLDEGKQSLTKLAAAWGGSG
SEAYQGVQQKWDATATELNNALQ
NLARTISEAGQAMASTEGNVTGMF
AKLAAALEHHHHHH.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.