Recombinant Human Osteocrin|2μg/10μg/1mg|PRO-420-上海希美化学有限公司

Recombinant Human Osteocrin

参考价：面议

供货周期：

一周

品牌：

Prospect Biosystems

规格：

2μg/10μg/1mg

货号：

PRO-420

CAS号：

产品详情

Osteocrin Human
Osteocrin Human Recombinant
Catalog No. PRO-420

Synonyms:
Osteocrin, Musclin, OSTN.

Introduction:
Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4 kDa. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dose- dependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.

Description:
The recombinant Human Osteocrin is produced with N-terminal fusion of His Tag. The Human Osteocrin His-Tagged Fusion Protein is 13.6 kDa containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues – His Tag (underlined).
MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPLDRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG

Source:
Escherichia Coli.

Physical Appearance:
Filtered White lyophilized (freeze-dried) powder.

Formulation:
Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer, pH 4.0.

Solubility:
It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Stability:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Purity:
Greater than 95% as determined by SDS-PAGE.

Applications:
Western blotting

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Recombinant Human Osteocrin由上海希美化学有限公司为您提供，货号PRO-420，规格：2μg/10μg/1mg，CAS号：，如您想了解更多关于Recombinant Human Osteocrin价格、Recombinant Human Osteocrin结构式、批发、用途等信息，欢迎咨询。除供应Recombinant Human Osteocrin外，还可为您提供重组人波形蛋白(Recombinant Human Vimentin)、Recombinant Human Matlose Binding Protein(MBP)等试剂，公司有专业的客户服务团队，是您值得信赖的合作伙伴，上海希美化学客户服务电话，售前、售后均可联系。