供货周期: | 一周 |
品牌: | Prospect Biosystems |
规格: | 10μg/50μg/1mg |
货号: | PRO-415 |
CAS号: |
PDX1 Human
Pancreatic Duodenal Homeobox-1 Human Recombinant
Catalog No. PRO-415
Synonyms:
Pancreas/duodenum homeobox protein 1, PDX-1, Insulin promoter factor 1, IPF-1, Islet/duodenum homeobox-1, IDX-1, Somatostatin-transactivating factor 1, STF-1, Insulin upstream factor 1, IUF-1, Glucose-sensitive factor, GSF, PDX1, IPF1, IUF1, MODY4.
Introduction:
PDX1 is a transcription factor which is expressed in beta and delta cells of the islets of Langerhans and in scattered endocrine cells of the duodenum. Pancreatic Duodenal Homeobox-1 activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. PDX1 is particularly involved in glucose-dependent regulation of insulin gene transcription. Furthermore, PDX1 plays a key part in the development of the pancreas and islet cell ontogeny. As a result homozygous interference with the gene in humans and mice results in pancreatic agenesis. The result of heterozygous mutations in PDX1 gene is impaired glucose tolerance and symptoms of diabetes as seen in MODY4 and late-onset Type II (non-insulin-dependent) diabetes mellitus. PDX1 binds preferentially the DNA motif 5'-[ct]taat[tg]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, IPF1 is required for maintaining the hormone-producing phenotype of the beta-cell. In adults PDX1 expression is increased in the pancreas duct cells that have been induced to proliferate and differentiate to form new islets. Deficiencies in pancreatic PDX1 could therefore contribute to Type II diabetes by affecting compensatory mechanisms that boost the rate of beta-cell neogenesis to meet the increased insulin secretory demand.
Description:
Pancreatic Duodenal Homeobox-1 Human Recombinant, produced in E. coli, is a 30.64kDa protein containing 283 amino acids.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized powder of liquid in PBS, pH 7.4.
Solubility:
It is recommended to reconstitute the lyophilized PDX1 in sterile PBS, 10% glycerol to prepare a stock of 100 μg/ml.
Stability:
Recombinant Human PDX1 although stable at 25°C 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
The activity of human PDX-1 is determined by the ability to stimulate NeuroD1 gene expression in WB cells and binding to insulin promoter be EMSA assay.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» PDX-1 antibody
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
3-D Life Dextran-PEG Hydrogel Kit
3-D Life Dextran-CD Hydrogel Kit(3D 细胞培养 葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒)
3-D水凝胶PEG-Link(聚乙二醇交联剂)(3D细胞培养 PEG 交联物(非降解))
3-D Life CD-Link(3D细胞培养 CD 交联物(可细胞降解))
3-D Life Maleimide-Dextran Set(3D 细胞培养葡聚糖马来酰亚胺聚合物)
3D 细胞培养RGD多肽(粘附剂)3-D Life RGD Peptide
3-D Life Scrambled RGD Peptide
Pig C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Mouse C-type lectin domain family 5 member A(CLEC5A)ELISA kit
Human C-type lectin domain family 5 member A(CLEC5A)ELISA kit
无形体IgM免疫荧光试剂盒
牛甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
鸡甘油醛-3-磷酸脱氢酶(GAPDH)ELISA kit
详细地址
关注
拨打电话
留言咨询