| 供货周期： | 一周 |
| 品牌： | Prospect Biosystems |
| 规格： | 5μg/20μg/1mg |
| 货号： | PRO-333 |
| CAS号： |
TXN1 Yeast
Thioredoxin Yeast Recombinant
Catalog No. PRO-333
Synonyms:
Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, TRX1, TRX2, YLR043C.
Introduction:
Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Description:
Thioredoxin Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Lyophilized Powder.
Formulation:
Each mg of protein contains 20mM phosphate buffer pH 7.4.
Solubility:
It is recommended to reconstitute the lyophilized TRX in sterile 18MΩ-cm H2O.
Stability:
TRX although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5).
The specific activity was found to be 3IU/mg.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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» TXN1 E.Coli
» TXN1 Human
» TXN2 Human
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