以色列进口小鼠白介素-6/IL 6 Mouse 重组小鼠白介素-6/IL 6 Mouse
SynonymsIFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.IntroductionInterleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.DescriptionInterleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.
The IL-6 is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationLyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.SolubilityIt is recommended to reconstitute the lyophilized Mouse Il-6 in sterile 18M?-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.PurityGreater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.Amino acid sequenceFPTSQVRRGD FTEDTTPNRP VYTTSQVGGL ITHVLWEIVE MRKELCNGNS DCMNNDDALA ENNLKLPEIQ RNDGCYQTGY NQEICLLKIS SGLLEYHSYL EYMKNNLKDN KKDKARVLQR DTETLIHIFN QEVKDLHKIV LPTPISNALL TDKLESQKEW LRTKTIQFIL KSLEEFLKVT LRSTRQT.Biological ActivityThe ED50 as determined by the dose-dependant stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is < 0.02 ng/ml, corresponding to a specific activity of > 50,000,000 units/mg.ReferencesTitle:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.
Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .
Link:http://www.fasebj.org/content/24/8/2869.fullUsageProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prodigiosin/灵菌红素的PubChem识别号5351169
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