产品详情
重组人二甲基精氨酸二甲胺水解酶1/DDAH1 Human
产品编号:ENZ-014
产品规格:5ug/20ug/1mg
Catalogue NumberENZ-014SynonymsDDAH, DDAH-1, Dimethylargininase-1, dimethylargininase-1, Dimethylarginine Dimethylaminohydrolase 1.IntroductionDimethylarginine dimethylaminohydrolase 1, is a part of the Dimethylarginine Dimethylaminohydrolase gene family. DDAH1 participates in nitric oxide generation by regulating cellular concentrations of methylarginines, that in turn inhibit nitric oxide synthase activity. Deficiency of DDAH1 results in ADMA (asymmetric dimethylarginine) increase and a decrease in cGMP generation.DescriptionDDAH1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-285a.a.) and having a molecular mass of 33.5kDa.
DDAH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical AppearanceSterile Filtered clear solution.FormulationThe DDAH1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 50mM NaCl and 10% glycerol.Amino Acid SequenceMGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDSPurityGreater than 95% as determined by SDS-PAGE.StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.UsageProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.