供货周期: | 一周 |
品牌: | Prospec-Tany |
规格: | 5μg/20μg/1mg |
货号: | CHM-343 |
CAS号: |
Catalogue Number | CHM-343 |
Synonyms | Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA. |
Introduction | Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-a from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17. |
Description |
Macrophage Inflammatory Protein-1 alpha Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. The MIP-1a is purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | The protein was lyophilized from 1 mg/ml solution containing no additives. |
Solubility | It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
Stability |
Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity |
Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Tyr-Gly-Ala. |
Biological Activity | The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml corresponding to a Specific Activity of 16,667IU/mg. |
Protein content |
Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of MIP-1a as a Reference Standard. |
Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
相关产品
人胃泌素(Gastrin)ELISA试剂盒Human Gastrin ELISA Kit
人胃动素(MTL)ELISA试剂盒Human MTL ELISA Kit
人胃蛋白酶(PG)ELISA试剂盒Human Pepsin ELISA Kit
人维生素D3(VD3)ELISA试剂盒Human VD3 ELISA kit
人维生素B1(VB1)ELISA试剂盒Human Vitamin B1 ELISA kit
人晚期氧化蛋白产物(AOPP)ELISA试剂盒Human AOPP ELISA Kit
人晚期糖基化终末产物受体(RAGE/AGER)ELISA试剂盒Human RAGE/AGER ELISA Kit
人晚期糖基化终末产物(AGEs)ELISA试剂盒Human AGEs ELISA Kit
人唾液酸(SA)ELISA试剂盒Human,SA ELISA Kit
人褪黑素(MT)ELISA试剂盒Human,MT ELISA Kit
人透明质酸(HA)ELISA试剂盒Human,HA ELISA Kit
人同型半胱氨酸(Hcy)ELISA试剂盒Human,Hcy ELISA试剂盒
人铁蛋白(FE)ELISA试剂盒Human,FE ELISA试剂盒
人天门冬氨酸氨基转移酶(AST)ELISA试剂盒Human AST ELISA试剂盒
人糖原磷酸化酶同工酶BB(GP-BB)ELISA试剂盒Human GP-BB ELISA试剂盒
详细地址
关注
拨打电话
留言咨询