Activo-P14半自动多肽合成仪

多功能正压型固相萃取仪

简介：介绍MULTI-SPE M08正压型固相萃取仪。适用于1、3、6、10、30、60 mL SPE柱。可进行单柱及双柱萃取。60 mL以内样品一次载入，简单方便。每个通道独立流速调节。最大收集体积可达100 mL。

建立等离子共振/质谱矩阵芯片平台

简介：Protein microarrays are the format of choice for high throughput, high content protein interaction analysis. In most of the array formats reporter molecules are used in multistep detection of the protein interactions. Among the few exixiting lable-free detection approaches, surface plasmon resonance(SPR) and mass spectormetry (MS) stand out as most promising for utilization in protein microarrays, albeit both have been used only sporadically for high content protein arrays. Shown here for the first time is the combination of SPR and MS detections on a single high content protein microarry. Antibodies to five human plasma proteins were arrayed in a 10 x 10 spot arrangement on a chemically activated gold coated glass chip. Binding of proteins to their corresponding antibodies was monitored via SPR imaging across the entire surface of the chip. Following protein affinity retrieval, the chip was overlaid with MALDI matrix and MS analyzed, producing protein-specific mass spectra from distinct spots on the array. The SPR/MS dual detection is well suited for high content protein microarrays and comprehensive protein analysis-from quantitative assessment of the protein concentration to detection of structural protein variants arising from genetic variations and postexpression procedding.

应用SPRimager II及SpotReady芯片监测DNA-DNA相互作用

简介：In this experment, two thiol-modified DNA oligomers are covalently immobilized on the gold surface of SpotReady chips. Specific binding of DNA complementay to one of the oligomers is monitored on the SPRimager II. In addition to DNA oligomers, this probe immobilization protocol can be used to attach any thiol modified probe to gold, such as thiolated RNA oligomers or peptides synthesized with a terminal cysteine. As an alternative, oligonucleotide arrays can instead be prepared on s streptavidin surface using biotinylated oligomers as probes.