NEPA21高效基因转染系统
---适用于体外(In Vitro)和活体(In Vivo)

    NEPA GENE公司专业研发、生产细胞电转染仪及电融合仪等，其生产CUY21系列电转染仪在研究领域中久负盛名，已被数百篇文献引用，其中不乏高水平杂志的文章，如Nature、Cell、PNAS、Genes & Dvelopment等。
    2011年，NEPA GENE公司推出新一代全能型NEPA21高效基因转染系统。与传统的电转仪相比，NEPA21采用全新设计的电转程序，特别适用于难转染细胞、离体组织或动物活体的转染。配合独有的电压衰减（Voltage Decay）设计，NEPA21可在获得高转染效率的同时，提高细胞存活率。

NEPA21高效基因转染系统应用范围十分广泛：
    ► 对真核细胞进行快速、高效、高存活率的基因转染，特别针对难转染的细胞，如原代细胞、神经细胞、干细胞、悬浮细胞等提供优化的实验方案
    ► 对贴壁状态的细胞直接进行转染，无需经过细胞消化、悬浮的过程，增加了细胞存活率和实验的简便性
    ► 对离体的组织或器官进行转染（Ex Vivo Transfection）
    ► 进行活体动物的基因转染（In Vivo Transfection）

NEPA21高效基因转染系统的优点：

    • 全新设计的电转程序

    • 特别适用于难转染细胞、离体组织或动物活体的转染

    • 适用于DNA和RNA（如siRNA等）转染

    • 高转染效率、高细胞存活率

    • 电转程序各项参数可见、可调，适用性广

    • 不需要特殊的转染试剂盒辅助，运维成本低



应用范围：
    ♦ 悬浮转染
      适用范围：原代细胞、干细胞、以及各种难转染细胞（如免疫细胞、血液细胞等）
    ♦ 贴壁转染
      适用范围：可以直接对贴壁细胞进行转染
    ♦ 离体组织转染
      适用范围：组织切片、脑切片、器官、胚胎等
    ♦ 活体转染
      适用范围：大脑、视网膜、肌肉、皮肤、肝脏、肾脏、睾丸等
    ♦ 植物细胞转染：
      如未去除细胞壁的衣藻的电转:http://www.instrument.com.cn/netshow/SH102009/news_91877.htm

国内电转DEMO结果（节选）：
    原代神经元转染效果的新闻报道 http://www.instrument.com.cn/show/news/20120705/079950.shtml
    水牛细胞转染效果的新闻报道 http://www.instrument.com.cn/show/news/20120426/077389.shtml
    U87 HELA转染效果的新闻报道 http://www.instrument.com.cn/show/news/20120320/075758.shtml 
    鸡原代输卵管上皮细胞转染效果的新闻报道http://www.instrument.com.cn/show/news/20120827/081967.shtml

NEPA21作为明星产品亮相各展会：
    国际转基因技术协会第十一届世界大会:http://www.instrument.com.cn/netshow/SH102009/news_92901.htm
    2012第五届再生医学和干细胞大会:http://www.instrument.com.cn/netshow/SH102009/news_86043.htm
    厦门-细胞探索完美方案之应用交流会:http://www.instrument.com.cn/netshow/SH102009/news_86039.htm
    国际转基因技术协会第十一届世界大会:http://www.instrument.com.cn/netshow/SH102009/news_81208.htm

引用文献:
NEPA21
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CUY21
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世界上首位报道用电转染法进行小鼠胚胎大脑活体转染（Neuroscience.2001;103(4):865-72）的科学家庆应大学Keio University的Dr H Tabata亲自动手演示胚胎活体转染的操作方法，请点击观看视频：

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