Step | Task | Time | Action | Buffer | Memo | Execute x times |
1 | Set temp | Off | Temperature to RT | |||
2 | Incubate | 00:05 | Wash | PBST | Wash membrane | X2 |
3 | Set temp | ON, 10℃ | Temperature 10℃ | |||
4 | Incubate | 01:00 | Blocking | Block solution | Blocking | |
5 | Incubate | 02:00 | AB-step | Primary antibody | Primary antibody | |
6 | Incubate | 00:05 | Wash | PBST | Wash membrane | X4 |
7 | Incubate | 00:30 | Sec. antibody | Second antibody | Second antibody | |
8 | Set temp | Off | Temperature to RT | |||
9 | Wash | 00:05 | PBST wash | PBST | PBST wash | X10 |
10 | Ready to stain in dark room |
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