中药材和饮片含量测定>色谱法检测 | 耐士科技：使用Biotage Isolera快速纯化液相制备色谱从库拉索芦荟叶皮提取天然的磷酸二酯酶抑制剂by耐士科技-上海鑫欣生物科技有限公司

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应用领域	制药/生物制药
检测样本	中药材和饮片
检测项目	含量测定>色谱法
参考标准	GB/T 8****

The ethanolic extract of Aloe barbadensis Miller leaf skin showed inhibitory activity against phosphodiesterase-4D (PDE4D), which is a therapeutic target of inflammatory disease. Subsequent bioassay-guided fractionation led to the isolation of two new anthrones, 6′-Oacetyl- aloin B (9) and 6′-O-acetyl-aloin A (11), one new chromone, aloeresin K (8), together with thirteen known compounds. Their chemical structureswere elucidated by spectroscopic methods including UV, IR, 1D and 2DNMR, and HRMS. All of the isolateswere screened for their inhibitory activity against PDE4D using tritium-labeled adenosine 3′,5′-cyclic monophosphate (3H cAMP) as substrate. Compounds 13 and 14were identified as PDE4D inhibitors,with their IC50 values of 9.25 and 4.42μM, respectively. These achievements can provide evidences for the use of A. barbadensis leaf skin as functional feed additives for anti-inflammatory purpose.

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