重组人白介素-3 Yeast(Human IL-3 Yeast)

IL 3 Human, Yeast
Interleukin-3 Human Recombinant, Yeast
Catalog No. CYT-588 
Synonyms:
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Introduction:
IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Description:
Interleukin-3 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton.
The recombinant human IL-3 migrates as a broad band between 15 and 22 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the e.coli IL-3 that has a predicted molecular mass of 15.1 kDa. IL-3 has N-linked and possibly O-linked oligosaccharides.

Source:
Saccharomyces cerevisiae.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Phosphate buffer pH-6.3.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
A P M T Q T T P L K T S W V N C S N M I D E I I T H L K Q P P L P L L D F N N L N G E D Q D I L M E N N L R R P N L E A F N R A V K S L Q N A S A I E S I L K N L L P C L P L A T A A P T R H P I H I K D G D W N E F R R K L T F Y L K T L E N A Q A Q Q T T L S L A I F.

Biological Activity:
The ED50 of IL-3 is typically 0.1- 0.4 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
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