重组人神经营养因子3|Human NT-3|2μg|CYT-257-上海高创化学科技有限公司

产品详情

Prospec (蛋白质专家) 是一家领先的生物科技公司，有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子，生长因子，趋化因子，激素，酶，病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利，所以Prospec以生产成本低，生产效率高，产品纯度高，有竞争力的价格，使Prospec产品在市场上立于不败之地（占有很大优势）。
Prospec为满足顾客的需要，还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构，政府研究部门，生物科技和药物公司以及医院和参照实

Synonyms

Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3.

Introduction

NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

Description

Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.
The NT-3 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT.

Biological Activity

The ED50 as determined by the dose-dependant induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50ng/ml, corresponding to a Specific Activity of 20,000-50,000IU/mg.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.

References

1.Title:Generation of Dopamine Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant^†^‡.
Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press.
Link:http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full

2.Title:Mash1 and Neurogenin 2 Enhance Survival and Differentiation of Neural Precursor Cells After Transplantation to Rat Brains via Distinct Modes of Action.
Publication:0Received 22 May 2008; Accepted 8 August 2008; Published online 9 September 2008.
Molecular Therapy (2008) 16 11, 1873–1882 doi:10.1038/mt.2008.189
Link:http://www.nature.com/mt/journal/v16/n11/full/mt2008189a.html

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

供货周期：	一周
品牌：	Prospec-Tany
规格：	2μg
货号：	CYT-257
CAS号：