重组人半乳糖凝集素3/Human LGALS3|50μg|CYT-606-上海高创化学科技有限公司

重组人半乳糖凝集素3/Human LGALS3

参考价：¥2000

供货周期：

一周

品牌：

Prospec-Tany

规格：

50μg

货号：

CYT-606

CAS号：

产品详情

重组人半乳糖凝集素3/Human LGALS3

Galectin-3 Human Recombinant

产品详情：

CATALOGUE NUMBER

CYT-606

SYNONYMS

Galectin-3, GAL3, MAC2, CBP35, GALB, GALIG, LGALS2, LGALS3, Galactose-specific lectin 3, Mac-2 antigen, IgE-binding protein, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Laminin-binding protein, Lectin L-29, L-31, Galactoside-binding protein, GALBP.

INTRODUCTION

Galectin-3 mediates with the alpha-3, beta-1 integrin the stimulation by cspg4 of endothelial cells migration. Galectin-3 plays an necessary part during the acquisition of vasculogenic mimicry and angiogenic properties associated with melanoma progression. LGALS3 overexpression is highly expressed in early stages of papillary carcinoma, and its expression intensity declines during tumor progression. Serum levels of LGALS3 are high in patients with thyroid malignancy but there is considerable overlap in serum LGALS3 concentrations between those with benign and malignant nodular thyroid disease. LGLAS3 takes part as an immune regulator to inhibit T-cell immune responses and promote tumor growth, as a result providing a new mechanism for tumor immune tolerance.

DESCRIPTION

LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 249 amino acids and having a molecular mass of 26kDa.
The LGALS3 is purified by proprietary chromatographic techniques.

SOURCE

Escherichia Coli.

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

The Galectin-3 protein was lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4 and 3mM DTT.

SOLUBILITY

It is recommended to reconstitute the lyophilized LGALS3 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

Lyophilized Galectin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

PURITY

Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC.

AMINO ACID SEQUENCE

ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI.

BIOLOGICAL ACTIVITY

The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 μg/ml.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.