供货周期: | 一周 |
品牌: | Prospec-Tany |
规格: | 2μg/10μg/1mg |
货号: | CYT-410 |
CAS号: |
Prospec (蛋白质专家) 是一家领先的生物科技公司,有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子,生长因子,趋化因子,激素,酶,病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利,所以Prospec以生产成本低,生产效率高,产品纯度高,有竞争力的价格,使Prospec产品在市场上立于不败之地(占有很大优势)。
Prospec为满足顾客的需要,还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构,政府研究部门,生物科技和药物公司以及医院和参照实
G CSF Mouse
Granulocyte-Colony Stimulating Factor Mouse Recombinant
Catalog No. CYT-410
Synonyms:
CSF3, MGI-1G, GM-CSF beta, Pluripoietin, G-CSF, GCSF.
Introduction:
Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. GCSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.
Description:
Granulocyte Colony Stimulating Factor Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 179 amino acids and having a molecular mass of 19 KD.
G-CSF is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
GCSF was lyophilized with no additives.
Solubility:
It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below
-18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Leu-Val.
Purity:
Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by SDS-PAGE.
Biological Activity:
The ED50 range=0.01-0.03 ng/mL, determined by the dose-dependant proliferation of mouse NFS- 60cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.
Protein content:
GCSF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.51 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of G-CSF as a Reference Standard.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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