供货周期: | 一周 |
品牌: | |
规格: | 2μg/10μg/1mg |
货号: | CYT-234 |
CAS号: |
Prospec (蛋白质专家) 是一家领先的生物科技公司,有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子,生长因子,趋化因子,激素,酶,病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利,所以Prospec以生产成本低,生产效率高,产品纯度高,有竞争力的价格,使Prospec产品在市场上立于不败之地(占有很大优势)。
Prospec为满足顾客的需要,还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构,政府研究部门,生物科技和药物公司以及医院和参照实
IFN b 1b Human
Interferon-Beta 1b Human Recombinant
Catalog No. CYT-234
Synonyms:
Leukocyte interferon, B cell interferon, Type I interferon, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.
Introduction:
Interferon-beta 1b has antiviral, antibacterial and anticancer activities.
Description:
Interferon beta 1b Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.
The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.
IFN beta-1b is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Formulation:
Lyophilized from 1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.
Solubility:
It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Interferon-beta 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNB 1b should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.
Biological Activity:
The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32 x 106 IU/mg.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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